Katherin Steffy1, Ganesan Shanthi2, Anson S Maroky3, Sachidanandan Selvakumar4. 1. Division of Microbiology, Rajah Muthiah Medical College, Annamalai University, Chidambaram 608002, Tamil Nadu, India. Electronic address: katherinsteffy88@gmail.com. 2. Division of Microbiology, Rajah Muthiah Medical College, Annamalai University, Chidambaram 608002, Tamil Nadu, India. 3. Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Chidambaram 608002, Tamil Nadu, India. 4. Department of Zoology, Faculty of Science, Annamalai University, Chidambaram 608002, Tamil Nadu, India.
Abstract
BACKGROUND: Increased incidence of Multi-drug resistance in microorganisms has become the greatest challenge in the treatment of Diabetic Foot Ulcer (DFU) and urges the need of a new antimicrobial agent. In this study, we determined the bactericidal effects of ZnO nanoparticles (ZnO NPs) green synthesized from Aristolochia indica against Multi-drug Resistant Organisms (MDROs) isolated from pus samples of DFU patients attending in a tertiary care hospital in South India. METHODS: ZnO NPs were characterized by UV-vis-DRS spectroscopy, Atomic Force Microscopy (AFM), Transmission Electron Microscopy (TEM) and for its zeta potential value. MIC/MBC assays were performed to determine bactericidal or bacteriostatic effects. Time-kill assays, Protein leakage and Flow cytometric analysis evaluated bacterial cell death at 1x MIC and 2x MIC concentrations of ZnO NPs. RESULTS: ZnO NPs of size 22.5nm with a zeta potential of -21.9±1mV exhibited remarkable bactericidal activity with MIC/MBC ranging from 25 to 400μg/ml with a significant reduction in viable count from 2h onwards. Protein leakage and Flow cytometric analysis confirmed bacterial cell death due to ZnO NPs. CONCLUSION: This study concluded that green synthesis protocol offers reliable, eco-friendly approach towards the development of antimicrobial ZnO NPs to combat antibiotic drug resistance.
BACKGROUND: Increased incidence of Multi-drug resistance in microorganisms has become the greatest challenge in the treatment of Diabetic Foot Ulcer (DFU) and urges the need of a new antimicrobial agent. In this study, we determined the bactericidal effects of ZnO nanoparticles (ZnO NPs) green synthesized from Aristolochia indica against Multi-drug Resistant Organisms (MDROs) isolated from pus samples of DFU patients attending in a tertiary care hospital in South India. METHODS:ZnO NPs were characterized by UV-vis-DRS spectroscopy, Atomic Force Microscopy (AFM), Transmission Electron Microscopy (TEM) and for its zeta potential value. MIC/MBC assays were performed to determine bactericidal or bacteriostatic effects. Time-kill assays, Protein leakage and Flow cytometric analysis evaluated bacterial cell death at 1x MIC and 2x MIC concentrations of ZnO NPs. RESULTS:ZnO NPs of size 22.5nm with a zeta potential of -21.9±1mV exhibited remarkable bactericidal activity with MIC/MBC ranging from 25 to 400μg/ml with a significant reduction in viable count from 2h onwards. Protein leakage and Flow cytometric analysis confirmed bacterial cell death due to ZnO NPs. CONCLUSION: This study concluded that green synthesis protocol offers reliable, eco-friendly approach towards the development of antimicrobial ZnO NPs to combat antibiotic drug resistance.
Authors: Miryam M Luzala; Claude K Muanga; Joseph Kyana; Justin B Safari; Eunice N Zola; Grégoire V Mbusa; Yannick B Nuapia; Jean-Marie I Liesse; Christian I Nkanga; Rui W M Krause; Aistė Balčiūnaitienė; Patrick B Memvanga Journal: Nanomaterials (Basel) Date: 2022-05-27 Impact factor: 5.719
Authors: Marco Ruggeri; Barbara Vigani; Cinzia Boselli; Antonia Icaro Cornaglia; Daniele Colombo; Rita Sànchez-Espejo; Elena Del Favero; Narcisa Mandras; Janira Roana; Lorenza Cavallo; Laura Cantù; Cesar Viseras; Silvia Rossi; Giuseppina Sandri Journal: Mater Today Bio Date: 2022-09-07