Literature DB >> 2914867

Translation and processing of Bacillus amyloliquefaciens extracellular RNase.

C J Paddon1, N Vasantha, R W Hartley.   

Abstract

Bacillus amyloliquefaciens extracellular RNase has been previously cloned and expressed in Bacillus subtilis. Site-specific mutagenesis experiments have identified codon -39 as the start site of translation. We have determined the primary signal peptide cleavage site of preprobarnase and propose a pathway for the conversion of probarnase to mature barnase.

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Year:  1989        PMID： 2914867      PMCID： PMC209718          DOI： 10.1128/jb.171.2.1185-1187.1989

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

1.  Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease.

Authors:  R W Hartley
Journal:  J Mol Biol       Date:  1988-08-20       Impact factor: 5.469

2.  Amino-acid sequence of extracellular ribonuclease (barnase) of Bacillus amyloliquefaciens.

Authors:  R W Hartley; E A Barker
Journal:  Nat New Biol       Date:  1972-01-05

3.  Production and purification of the extracellular ribonuclease of Bacillus amyloliquefaciens (barnase) and its intracellular inhibitor (barstar). II. Barstar.

Authors:  R W Hartley; D L Rogerson; J R Smeaton
Journal:  Prep Biochem       Date:  1972

4.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors:  W N Burnette
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

5.  Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.

Authors:  F Sanger; A R Coulson; B G Barrell; A J Smith; B A Roe
Journal:  J Mol Biol       Date:  1980-10-25       Impact factor: 5.469

6.  Site-directed mutagenesis of cytochrome c shows that an invariant Phe is not essential for function.

Authors:  G J Pielak; A G Mauk; M Smith
Journal:  Nature       Date:  1985 Jan 10-18       Impact factor: 49.962

7.  Cloning, sequencing and transcription of an inactivated copy of Bacillus amyloliquefaciens extracellular ribonuclease (barnase).

Authors:  C J Paddon; R W Hartley
Journal:  Gene       Date:  1985       Impact factor: 3.688

8.  Use of plasmid pTV1 in transposon mutagenesis and gene cloning in Bacillus amyloliquefaciens.

Authors:  R W Hartley; C J Paddon
Journal:  Plasmid       Date:  1986-07       Impact factor: 3.466

9.  Fusion of pro region of subtilisin to staphylococcal protein A and its secretion by Bacillus subtilis.

Authors:  N Vasantha; L D Thompson
Journal:  Gene       Date:  1986       Impact factor: 3.688

10.  Expression of Bacillus amyloliquefaciens extracellular ribonuclease (barnase) in Escherichia coli following an inactivating mutation.

Authors:  C J Paddon; R W Hartley
Journal:  Gene       Date:  1987       Impact factor: 3.688
  10 in total
  5 in total

1.  Effect of signal sequence alterations on export of levansucrase in Bacillus subtilis.

Authors:  T V Borchert; V Nagarajan
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

Review 2.  Protein secretion in Bacillus species.

Authors:  M Simonen; I Palva
Journal:  Microbiol Rev       Date:  1993-03

3.  The roles of signal peptide and mature protein in RNase (barnase) export from Bacillus subtilis.

Authors:  M Chen; V Nagarajan
Journal:  Mol Gen Genet       Date:  1993-06

4.  Refolding of barnase mutants and pro-barnase in the presence and absence of GroEL.

Authors:  T E Gray; J Eder; M Bycroft; A G Day; A R Fersht
Journal:  EMBO J       Date:  1993-11       Impact factor: 11.598

5.  Highly efficient generation of bacterial leaf blight-resistant and transgene-free rice using a genome editing and multiplexed selection system.

Authors:  Kun Yu; Zhiqiang Liu; Huaping Gui; Lizhao Geng; Juan Wei; Dawei Liang; Jian Lv; Jianping Xu; Xi Chen
Journal:  BMC Plant Biol       Date:  2021-04-24       Impact factor: 4.215
  5 in total

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