Literature DB >> 2914812

A rapid micro method for counting cells "in situ" using a fluorogenic alkaline phosphatase enzyme assay.

L I Huschtscha1, F C Lucibello, W F Bodmer.   

Abstract

A new method has been developed to count cells "in situ", based on a fluorogenic enzyme assay that measures the activity of alkaline phosphatase. Increasing cell number was shown to correlate closely with alkaline phosphatase activity and this relationship did not change with time in culture. The alkaline phosphatase assay (ALP assay) was able to estimate relative cell numbers over a range from about 10(4) to 5 X 10(5) for many cell types, including Hep-2, a derivative of HeLa, several human colorectal cell lines SW1222, SW837, LS174T and HT29, a normal human diploid cell strain MRC5 and a rodent line NIH-3T3. The ALP assay is rapid and efficient, making it a useful method for studying growth assays.

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Year:  1989        PMID： 2914812     DOI： 10.1007/BF02624419

Source DB:  PubMed          Journal:  In Vitro Cell Dev Biol        ISSN： 0883-8364


  8 in total

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  8 in total
  8 in total

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Authors:  A Martin; M Clynes
Journal:  In Vitro Cell Dev Biol       Date:  1991-03

Review 2.  Fluorogenic and chromogenic substrates used in bacterial diagnostics.

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Journal:  Microbiol Rev       Date:  1991-09

3.  Design and synthesis of fluorescent substrates for human tyrosyl-DNA phosphodiesterase I.

Authors:  Marc C Rideout; Amy C Raymond; Alex B Burgin
Journal:  Nucleic Acids Res       Date:  2004-08-27       Impact factor: 16.971

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Authors:  Z Parandoosh; C A Bogowitz; M P Nova
Journal:  In Vitro Cell Dev Biol Anim       Date:  1998 Nov-Dec       Impact factor: 2.416

5.  Comparison of 5 microplate colorimetric assays for in vitro cytotoxicity testing and cell proliferation assays.

Authors:  A Martin; M Clynes
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

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Journal:  J Cancer Res Clin Oncol       Date:  1995       Impact factor: 4.553

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Journal:  In Vitro Cell Dev Biol       Date:  1990-06

8.  Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro.

Authors:  Katarzyna Bednarska; Magdalena Klink; Zofia Sulowska
Journal:  Mediators Inflamm       Date:  2006       Impact factor: 4.711
  8 in total

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