Qianhui Qiu1, Yang Peng1,2,3, Zhenchao Zhu2, Zhuo Chen4, Chi Zhang2, Hsiao Hui Ong3, Kai Sen Tan3, Haiyu Hong5, Yan Yan3, Haoqi Huang6, Jing Liu3, Xianqing Li7, H N Nam8, N T N Dung8, Li Shi9, Qintai Yang10, Colin D Bingle11, De-Yun Wang3. 1. Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China. 2. Department of Otolaryngology Head and Neck Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, People's Republic of China. 3. Department of Otolaryngology, National University of Singapore, National University Health System, Singapore, Singapore. 4. Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, People's Republic of China. 5. Department of Otolaryngology-Head and Neck Surgery, the 5th Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People's Republic of China. 6. Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China. 7. Department of Otolaryngology Head and Neck Surgery, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, People's Republic of China. 8. Department of Otolaryngology, Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam. 9. Department of Otolaryngology, The Second Hospital of Shandong University, Jinan, People's Republic of, China. 10. Department of Otorhinolaryngology-Head and Neck Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, People's Republic of China. 11. Academic Unit of Respiratory Medicine, Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, United Kingdom.
Abstract
OBJECTIVE: Motile cilia impairment is a common condition in patients with chronically inflamed airways, such as is seen in nasal polyps (NPs). The mechanism underlying this pathogenic condition is complex and not fully understood. METHODS: We investigated the presence and localization of dynein axonemal heavy chain 5 (DNAH5) in motile cilia using immunofluorescence staining in paraffin-embedded nasal biopsies from NPs (n = 120) and inferior turbinate mucosa (n = 35) of healthy controls. We also performed single-cell staining on cytospin samples (NP = 5, control = 5). Three patterns of DNAH5 localization are defined, including pattern A (presence throughout the axoneme), pattern B (undetectable in the distal part of the axoneme), and pattern C (completely missing throughout the entire axoneme). We developed a semiquantitative scoring system for which 0 = (pattern A > 70%); 1 = (patterns A + B > 70%); and 2 = (pattern C ≥ 30%) in each high-power field (5 fields per sample). RESULTS: Based on our DNAH5 scoring system, the median (1st and 3rd quartile) score was 0.3 (0.2 and 0.4) for samples from controls, and 1.1 (0.6 and 1.6) for samples from NPs in paraffin specimens (P < 0.001). The DNAH5 score had a significant positive relationship with the Lund-Mackay computed tomography score (r = 0.329, P = 0.005) and was higher in patients with eosinophilic NPs (P = 0.006). For cytospin samples, the mean percentage of patterns A, B, and C were 74%, 14%, and 12% in controls, and 48%, 20%, and 32% in NPs, respectively. CONCLUSION: Our results suggest that the absence or mislocalization of DNAH5 from motile cilia is a common and potentially important pathological phenomenon in chronically inflamed airway epithelium. LEVEL OF EVIDENCE: NA. Laryngoscope, 128:E97-E104, 2018.
OBJECTIVE:Motile cilia impairment is a common condition in patients with chronically inflamed airways, such as is seen in nasal polyps (NPs). The mechanism underlying this pathogenic condition is complex and not fully understood. METHODS: We investigated the presence and localization of dynein axonemal heavy chain 5 (DNAH5) in motile cilia using immunofluorescence staining in paraffin-embedded nasal biopsies from NPs (n = 120) and inferior turbinate mucosa (n = 35) of healthy controls. We also performed single-cell staining on cytospin samples (NP = 5, control = 5). Three patterns of DNAH5 localization are defined, including pattern A (presence throughout the axoneme), pattern B (undetectable in the distal part of the axoneme), and pattern C (completely missing throughout the entire axoneme). We developed a semiquantitative scoring system for which 0 = (pattern A > 70%); 1 = (patterns A + B > 70%); and 2 = (pattern C ≥ 30%) in each high-power field (5 fields per sample). RESULTS: Based on our DNAH5 scoring system, the median (1st and 3rd quartile) score was 0.3 (0.2 and 0.4) for samples from controls, and 1.1 (0.6 and 1.6) for samples from NPs in paraffin specimens (P < 0.001). The DNAH5 score had a significant positive relationship with the Lund-Mackay computed tomography score (r = 0.329, P = 0.005) and was higher in patients with eosinophilic NPs (P = 0.006). For cytospin samples, the mean percentage of patterns A, B, and C were 74%, 14%, and 12% in controls, and 48%, 20%, and 32% in NPs, respectively. CONCLUSION: Our results suggest that the absence or mislocalization of DNAH5 from motile cilia is a common and potentially important pathological phenomenon in chronically inflamed airway epithelium. LEVEL OF EVIDENCE: NA. Laryngoscope, 128:E97-E104, 2018.
Authors: Kai Sen Tan; Rachel Liyu Lim; Jing Liu; Hsiao Hui Ong; Vivian Jiayi Tan; Hui Fang Lim; Kian Fan Chung; Ian M Adcock; Vincent T Chow; De Yun Wang Journal: Front Cell Dev Biol Date: 2020-02-25
Authors: Qianmin Chen; Kai Sen Tan; Jing Liu; Hsiao Hui Ong; Suizi Zhou; Hongming Huang; Hailing Chen; Yew Kwang Ong; Mark Thong; Vincent T Chow; Qianhui Qiu; De-Yun Wang Journal: Front Cell Dev Biol Date: 2020-12-21
Authors: Xiao-Xue Zi; Wei-Jie Guan; Yang Peng; Kai Sen Tan; Jing Liu; Ting-Ting He; Yew-Kwang Ong; Mark Thong; Li Shi; De-Yun Wang Journal: Front Genet Date: 2019-11-13 Impact factor: 4.599