Literature DB >> 2914218

Detection of African swine fever virus antibodies by immunoblotting assay.

M J Pastor1, M D Laviada, J M Sanchez-Vizcaino, J M Escribano.   

Abstract

An immunoblotting assay has been adapted to detect antibodies against African swine fever virus. The electrophoretic transfer of proteins and the immunoreaction conditions were optimized, using 4 mA/cm2 of current intensity and 10 micrograms of soluble cytoplasmic antigen of infected cells per strip. Filters of polyvinylidene difluoride showed the highest capacity for protein absorption, but nitrocellulose filters showed lower backgrounds. The specificity and the pattern of the proteins induced by African swine fever virus that react with the antisera were determined in immunoblotting assay, IP30 being the most reactive protein.

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Year:  1989        PMID： 2914218      PMCID： PMC1255524     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  7 in total

1.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors:  W N Burnette
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

3.  Proteins specified by African swine fever virus: V. Identification of immediate early, early and late proteins.

Authors:  J M Escribano; E Tabarés
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

4.  Detection of fish antibody against protein antigen of Aeromonas salmonicida by enzyme-linked immunosorbent assay using biotin-avidin system.

Authors:  H Kodama; A Honda; T Mikami; H Izawa
Journal:  Res Vet Sci       Date:  1987-07       Impact factor: 2.534

5.  Inhibition of African swine fever infection in the presence of immune sera in vivo and in vitro.

Authors:  F Ruiz Gonzalvo; M E Carnero; C Caballero; J Martínez
Journal:  Am J Vet Res       Date:  1986-06       Impact factor: 1.156

6.  Proteins specified by African swine fever virus. II. Analysis of proteins in infected cells and antigenic properties.

Authors:  E Tabarés; J Martinez; F Ruiz Gonzalvo; C Sánchez-Botija
Journal:  Arch Virol       Date:  1980       Impact factor: 2.574

7.  Antibody directed to determinants of a Moloney virus derived MCF GP70 recognizes a thymic differentiation antigen.

Authors:  D A Johnson; J H Elder
Journal:  J Exp Med       Date:  1983-11-01       Impact factor: 14.307
  7 in total
  9 in total

1.  Optimization and validation of recombinant serological tests for African Swine Fever diagnosis based on detection of the p30 protein produced in Trichoplusia ni larvae.

Authors:  D M Pérez-Filgueira; F González-Camacho; C Gallardo; P Resino-Talaván; E Blanco; E Gómez-Casado; C Alonso; J M Escribano
Journal:  J Clin Microbiol       Date:  2006-09       Impact factor: 5.948

2.  HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies.

Authors:  Huijun Zhao; Jiahui Ren; Shuya Wu; Haoran Guo; Yongkun Du; Bo Wan; Pengchao Ji; Yanan Wu; Guoqing Zhuang; Angke Zhang; Gaiping Zhang
Journal:  Appl Microbiol Biotechnol       Date:  2022-05-25       Impact factor: 5.560

3.  Recombinant antigen targets for serodiagnosis of African swine fever.

Authors:  Carmina Gallardo; Ana Luísa Reis; Gladys Kalema-Zikusoka; Joana Malta; Alejandro Soler; Esther Blanco; R M E Parkhouse; Alexandre Leitão
Journal:  Clin Vaccine Immunol       Date:  2009-05-06

4.  Antigenic properties and diagnostic potential of African swine fever virus protein pp62 expressed in insect cells.

Authors:  Carmina Gallardo; Esther Blanco; Javier M Rodríguez; Angel L Carrascosa; Jose Manuel Sanchez-Vizcaino
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

Review 5.  African swine fever: a global view of the current challenge.

Authors:  Ma Carmen Gallardo; Ana de la Torre Reoyo; Jovita Fernández-Pinero; Irene Iglesias; Ma Jesús Muñoz; Ma Luisa Arias
Journal:  Porcine Health Manag       Date:  2015-12-23

Review 6.  African Swine Fever Virus: An Emerging DNA Arbovirus.

Authors:  Natasha N Gaudreault; Daniel W Madden; William C Wilson; Jessie D Trujillo; Juergen A Richt
Journal:  Front Vet Sci       Date:  2020-05-13

Review 7.  African swine fever: A permanent threat to Indian pigs.

Authors:  Sharanagouda S Patil; Kuralayanapalya Puttahonnappa Suresh; Vikram Vashist; Awadhesh Prajapati; Bramhadev Pattnaik; Parimal Roy
Journal:  Vet World       Date:  2020-10-29

8.  K205R specific nanobody-horseradish peroxidase fusions as reagents of competitive ELISA to detect African swine fever virus serum antibodies.

Authors:  Angke Zhang; Shuya Wu; Xiaohong Duan; Huijun Zhao; Haoxin Dong; Jiahui Ren; Mingfang Zhang; Jiaji Li; Hong Duan; Gaiping Zhang
Journal:  BMC Vet Res       Date:  2022-08-20       Impact factor: 2.792

Review 9.  Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management.

Authors:  Dexin Zeng; Bingxu Qian; Yunfei Li; Kai Zong; Wanqing Peng; Kai Liao; Xiaofeng Yu; Juanjuan Sun; Xiaying Lv; Liu Ding; Manman Wang; Tingting Zhou; Yuan Jiang; Jinming Li; Feng Xue; Xiaodong Wu; Jianjun Dai
Journal:  Front Microbiol       Date:  2022-09-30       Impact factor: 6.064
  9 in total

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