Celia Oreja-Guevara1, Ulises Gómez-Pinedo2, Julia García-López3, Rafael Sánchez-Sánchez4, Roberto Valverde-Moyano5, Alberto Rabano-Gutierrez6, Jordi A Matías-Guiu7, Ines González-Suárez8, Jorge Matías-Guiu9. 1. Department of Neurology, Neuroscience Institute, Hospital Clínico San Carlos, Madrid, Spain and Laboratory of Neurobiology, Neuroscience Institute, Hospital Clínico San Carlos, C/ Prof. Martín Lagos, s/n, 28040 Madrid, Spain. Electronic address: orejacbn@gmail.com. 2. Department of Neurology, Neuroscience Institute, Hospital Clínico San Carlos, Madrid, Spain and Laboratory of Neurobiology, Neuroscience Institute, Hospital Clínico San Carlos, C/ Prof. Martín Lagos, s/n, 28040 Madrid, Spain. Electronic address: u.gomez.pinedo@gmail.com. 3. Department of Neurology, Neuroscience Institute, Hospital Clínico San Carlos, Madrid, Spain and Laboratory of Neurobiology, Neuroscience Institute, Hospital Clínico San Carlos, C/ Prof. Martín Lagos, s/n, 28040 Madrid, Spain. Electronic address: jdejuliagl@gmail.com. 4. Department of Anatomical Pathology, Servicio de Anatomía Patológica, Hospital Reina Sofía, Av. Menéndez Pidal, s/n, 14004 Córdoba, Spain. Electronic address: rafael.sanchez.sanchez.sspa@juntadeandalucia.es. 5. Department of Neurology, Servicio de Neurología, Hospital Reina Sofía, Av. Menéndez Pidal, s/n, 14004 Córdoba, Spain. Electronic address: Roberto.valverde-moyano.sspa@juntadeandalucia.es. 6. Fundación Centro Investigación Enfermedades Neurológicas (CIEN), C/ Valderrebollo, 5 Complejo Alzheimer PAU de Vallecas, 28031 Madrid, Spain. Electronic address: arabano@fundacioncien.es. 7. Department of Neurology, Neuroscience Institute, Hospital Clínico San Carlos, Madrid, Spain and Laboratory of Neurobiology, Neuroscience Institute, Hospital Clínico San Carlos, C/ Prof. Martín Lagos, s/n, 28040 Madrid, Spain. Electronic address: jordimatiasguiu@hotmail.com. 8. Department of Neurology, Neuroscience Institute, Hospital Clínico San Carlos, Madrid, Spain and Laboratory of Neurobiology, Neuroscience Institute, Hospital Clínico San Carlos, C/ Prof. Martín Lagos, s/n, 28040 Madrid, Spain. Electronic address: igonsua@gmail.com. 9. Department of Neurology, Neuroscience Institute, Hospital Clínico San Carlos, Madrid, Spain and Laboratory of Neurobiology, Neuroscience Institute, Hospital Clínico San Carlos, C/ Prof. Martín Lagos, s/n, 28040 Madrid, Spain. Electronic address: matiasguiu@gmail.com.
Abstract
INTRODUCTION: Neural stem cells (NSC) are located essentially in the subventricular zone (SVZ), subgranular zone (SGZ), and along the central canal of the spinal cord. These cells can proliferate in vitro and differentiate into neurons, oligodendrocytes, and astroglia, thus contributing to repair in multiple sclerosis (MS). We conducted a pathological study to analyse neurogenic response in a patient with Marburg variant MS. METHODS: We present the case of a 27-year-old immunocompetent patient with Marburg variant MS, a fulminant form of the disease. The condition lasted 20 days. Diagnosis was based on clinical symptoms and MRI showed demyelinating lesions located in subependymal areas and histopathological findings. Neurogenic niches (SVZ and dentate gyrus) were analysed by confocal microscopy using markers of proliferation (Ki-67, PCNA), neuroblasts (PSA-NCAM, DCX, Tuj1), stem cells (Nestin, GFAPδ, SOX2, PAX6, Musashi), astrocytes (GFAP, AQ4), oligodendrocytes (NG2, Olig), microglia and cell infiltrates (IBA-1, CD68, MHCII), and cell death (TUNEL). RESULTS: Expression of the markers GFAPδ, SOX2, and PAX6 in NSC was found to be very low. Likewise, markers of proliferation (Ki-67) and intermediate precursors (NG2) were also reduced. This lack of markers of the first stages of cell differentiation means that neurogenesis is inhibited even in very early stages of the disease. CONCLUSION: Inhibition of neurogenesis in our patient, which cannot be explained by the fulminant nature of his symptoms, may be related to inflammation and immune response. This finding may further our knowledge of repair mechanisms in MS.
INTRODUCTION: Neural stem cells (NSC) are located essentially in the subventricular zone (SVZ), subgranular zone (SGZ), and along the central canal of the spinal cord. These cells can proliferate in vitro and differentiate into neurons, oligodendrocytes, and astroglia, thus contributing to repair in multiple sclerosis (MS). We conducted a pathological study to analyse neurogenic response in a patient with Marburg variant MS. METHODS: We present the case of a 27-year-old immunocompetent patient with Marburg variant MS, a fulminant form of the disease. The condition lasted 20 days. Diagnosis was based on clinical symptoms and MRI showed demyelinating lesions located in subependymal areas and histopathological findings. Neurogenic niches (SVZ and dentate gyrus) were analysed by confocal microscopy using markers of proliferation (Ki-67, PCNA), neuroblasts (PSA-NCAM, DCX, Tuj1), stem cells (Nestin, GFAPδ, SOX2, PAX6, Musashi), astrocytes (GFAP, AQ4), oligodendrocytes (NG2, Olig), microglia and cell infiltrates (IBA-1, CD68, MHCII), and cell death (TUNEL). RESULTS: Expression of the markers GFAPδ, SOX2, and PAX6 in NSC was found to be very low. Likewise, markers of proliferation (Ki-67) and intermediate precursors (NG2) were also reduced. This lack of markers of the first stages of cell differentiation means that neurogenesis is inhibited even in very early stages of the disease. CONCLUSION: Inhibition of neurogenesis in our patient, which cannot be explained by the fulminant nature of his symptoms, may be related to inflammation and immune response. This finding may further our knowledge of repair mechanisms in MS.
Authors: Raymond A Sobel; Mary Jane Eaton; Prajakta Dilip Jaju; Eugene Lowry; Julian R Hinojoza Journal: J Neuropathol Exp Neurol Date: 2019-09-01 Impact factor: 3.685
Authors: Victor Oliveira Araújo; Paulo Filho Soares Marcelino; Samuel Ramos Rabelo; Fabio Antonio Serra de Lima Junior; Vitor Gil Gonçalves Teixeira; Roberto Rocha Eisele; Afonso Rocha Eisele; João Pedro Serenario Ferreira de Carvalho; Luciano Barroso de Albuquerque Filho; Sara Diógenes Peixoto de Medeiros; Leandro Freitas Oliveira; Julio Cesar Claudino Dos Santos; Euldes Mendes Júnior Journal: Radiol Case Rep Date: 2022-09-02