Masoumeh Pouladian1, Mazdak Ganjalikhani-Hakemi2, Fereshteh Alsahebfosul1, Vida Homayouni1, Sharifeh Khosravi3, Masoud Etemadifar4, Fariba Mazrouei1, Rasoul Salehi3. 1. Department of Immunology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran. 2. Department of Immunology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran. Electronic address: mghakemi@med.mui.ac.ir. 3. Department of Cellular & Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran. 4. Department of Neurosciences, Al-Zahra Hospital, Isfahan University of Medical Science, Isfahan, Iran.
Abstract
BACKGROUND: Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS) which initiated and mediated by autoreactive T helper1 cells directed against myelin antigens. One of T cell surface receptors is T cell immunoglobulin and mucin domain (TIM) family which its importance in immunology is recently discovered. These molecules have important immunological function by regulation of T effector cells. METHODS: In the present study, we analyzed the frequency of +4259A>C polymorphism in TIM-3 and -1637C>T polymorphism in TIM-1 gene in MS patients and healthy controls using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) method. RESULTS: We found that the polymorphism +4259A>C in exon 3 of the TIM-3 gene is associated with susceptibility to the MS (P = 0.029, OR (95%CI) = 1.841) but the other polymorphism, -1637T>C, in the promoter region of TIM-1 is not (p= 0.064). CONCLUSION: Our findings suggest that +4259A>C polymorphism in TIM-3 gene may be one of the important genetic factors associated with the MS susceptibility among Iranian populations.
BACKGROUND:Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS) which initiated and mediated by autoreactive T helper1 cells directed against myelin antigens. One of T cell surface receptors is T cell immunoglobulin and mucin domain (TIM) family which its importance in immunology is recently discovered. These molecules have important immunological function by regulation of T effector cells. METHODS: In the present study, we analyzed the frequency of +4259A>C polymorphism in TIM-3 and -1637C>T polymorphism in TIM-1 gene in MSpatients and healthy controls using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) method. RESULTS: We found that the polymorphism +4259A>C in exon 3 of the TIM-3 gene is associated with susceptibility to the MS (P = 0.029, OR (95%CI) = 1.841) but the other polymorphism, -1637T>C, in the promoter region of TIM-1 is not (p= 0.064). CONCLUSION: Our findings suggest that +4259A>C polymorphism in TIM-3 gene may be one of the important genetic factors associated with the MS susceptibility among Iranian populations.