Swapnil Bhisikar1, Jayashree Mondkar2, Swati Manerkar2, Sorabh Goel2, Desma D'Dsouza3, Sujata Baveja3. 1. Department of Neonatology, Lokmanya Tilak Municipal Medical College and Lokmanya Tilak Municipal and General Hospital, Room no 123, 1st Floor, College Building, Sion (West), Mumbai, 400022, India. swapbhi@gmail.com. 2. Department of Neonatology, Lokmanya Tilak Municipal Medical College and Lokmanya Tilak Municipal and General Hospital, Room no 123, 1st Floor, College Building, Sion (West), Mumbai, 400022, India. 3. Department of Microbiology, Lokmanya Tilak Municipal Medical College and Lokmanya Tilak Municipal and General Hospital, Mumbai, India.
Abstract
OBJECTIVE: To identify, if Dornic acid test done on human milk bank sample is as effective as doing routine culture, both pre and post pasteurization. METHODS: The authors analyzed 477 samples, both pre and post pasteurization. Dornic acid measurement was performed by using N/9 NaOH and titrated to get dornicity of the sample. Senstivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) at different Dornic acid levels were calculated. To find diagnostic power of this study, ROC curve was prepared. Dornic acid for paired pre and post pasteurization were noted and analysed for statistical significance. RESULTS: A significant growth was seen in 21% (98/477) samples with coagulase negative staphylococci and gram negative organisms being the major contaminants seen prior to pasteurization. In the index study, Dornic acidity ≥4°D had a sensitivity of 98% but very low specificity of just over 10%. However, 8°D had 92% specificity and acceptable sensitivity of 72% in comparison to gold standard microbiological criteria, making it a good test for analyzing the quality of milk before pasteurization. CONCLUSIONS: Dornic acid test at 8°D has a very high specificity with acceptable sensitivity in comparison to dornicity at 4°D. It can be used as a simple method to select better quality of milk sample prior to pasteurization.
OBJECTIVE: To identify, if Dornic acid test done on human milk bank sample is as effective as doing routine culture, both pre and post pasteurization. METHODS: The authors analyzed 477 samples, both pre and post pasteurization. Dornic acid measurement was performed by using N/9 NaOH and titrated to get dornicity of the sample. Senstivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) at different Dornic acid levels were calculated. To find diagnostic power of this study, ROC curve was prepared. Dornic acid for paired pre and post pasteurization were noted and analysed for statistical significance. RESULTS: A significant growth was seen in 21% (98/477) samples with coagulase negative staphylococci and gram negative organisms being the major contaminants seen prior to pasteurization. In the index study, Dornic acidity ≥4°D had a sensitivity of 98% but very low specificity of just over 10%. However, 8°D had 92% specificity and acceptable sensitivity of 72% in comparison to gold standard microbiological criteria, making it a good test for analyzing the quality of milk before pasteurization. CONCLUSIONS:Dornic acid test at 8°D has a very high specificity with acceptable sensitivity in comparison to dornicity at 4°D. It can be used as a simple method to select better quality of milk sample prior to pasteurization.
Entities:
Keywords:
Dornic acid; Human milk bank; Pasteurization
Authors: Sertac Arslanoglu; Enrico Bertino; Paola Tonetto; Giuseppe De Nisi; Amalia Maria Ambruzzi; Augusto Biasini; Claudio Profeti; Maria Rita Spreghini; Guido E Moro Journal: J Matern Fetal Neonatal Med Date: 2010-09
Authors: Sara Vázquez-Román; Nadia Raquel Garcia-Lara; Diana Escuder-Vieco; Fernando Chaves-Sánchez; Javier De la Cruz-Bertolo; Carmen Rosa Pallas-Alonso Journal: Breastfeed Med Date: 2012-06-25 Impact factor: 1.817