Hiroaki Matsunaga1, Misaki Iwashita1, Takanori Shinjo1, Akiko Yamashita1, Mitsudai Tsuruta1, Shoichiro Nagasaka2, Ataru Taniguchi3, Mitsuo Fukushima4, Naoya Watanabe5, Fusanori Nishimura6. 1. Section of Periodontology, Kyushu University Faculty of Dental Science, Fukuoka, Japan. 2. Division of Diabetes, Metabolism and Endocrinology, Showa University Fujigaoka Hospital, Yokohama, Japan. 3. Division of Diabetes and Endocrinology, Kyoto Preventive Medical Center, Kyoto, Japan. 4. Preemptive Medicine and Lifestyle-related Disease Research Center, Kyoto University Hospital, Kyoto, Japan. 5. Health Care and Promotion Center, Yodogawa Christian Hospital, Osaka, Japan. 6. Section of Periodontology, Kyushu University Faculty of Dental Science, Fukuoka, Japan. Electronic address: fusanori@dent.kyushu-u.ac.jp.
Abstract
OBJECTIVES: It is well-known that the complement system plays an essential role in host immunity. Observational studies have indicated that complement system-related molecules such as complement factor B (CfB) and other components are correlated with obesity and/or insulin resistance parameters. In this study, we investigated the role of adipocyte-derived CfB in adipose tissue metabolism. METHODS: We investigated the expression level of complement system-related genes in adipocytes. To understand the role of CfB in adipocyte, we performed Cfb overexpression in 3T3-L1 preadipocytes and generated adipocyte-specific Cfb transgenic mice. RESULTS: Cfb expression was markedly enhanced in 3T3-L1 adipocytes co-cultured with macrophages following endotoxin stimulation. In Cfb-overexpressing cells, the expression of adipocyte differentiation/maturation-related genes encoding peroxisome proliferator-activated receptor γ (Pparγ), adipocyte Protein 2 and perilipin was significantly enhanced. Cfb transgenic mice showed a marked increase in the expression of genes encoding Pparγ, perilipin, sterol regulatory element-binding protein 1 c, and Cd36 in the subcutaneous adipose tissue. CONCLUSIONS: CfB plays a crucial role in late-phase of adipocyte differentiation and subsequent lipid droplet formation.
OBJECTIVES: It is well-known that the complement system plays an essential role in host immunity. Observational studies have indicated that complement system-related molecules such as complement factor B (CfB) and other components are correlated with obesity and/or insulin resistance parameters. In this study, we investigated the role of adipocyte-derived CfB in adipose tissue metabolism. METHODS: We investigated the expression level of complement system-related genes in adipocytes. To understand the role of CfB in adipocyte, we performed Cfb overexpression in 3T3-L1 preadipocytes and generated adipocyte-specific Cfbtransgenic mice. RESULTS:Cfb expression was markedly enhanced in 3T3-L1 adipocytes co-cultured with macrophages following endotoxin stimulation. In Cfb-overexpressing cells, the expression of adipocyte differentiation/maturation-related genes encoding peroxisome proliferator-activated receptor γ (Pparγ), adipocyte Protein 2 and perilipin was significantly enhanced. Cfbtransgenic mice showed a marked increase in the expression of genes encoding Pparγ, perilipin, sterol regulatory element-binding protein 1 c, and Cd36 in the subcutaneous adipose tissue. CONCLUSIONS:CfB plays a crucial role in late-phase of adipocyte differentiation and subsequent lipid droplet formation.
Authors: Nicholas J Carruthers; Clarissa Strieder-Barboza; Joseph A Caruso; Carmen G Flesher; Nicki A Baker; Samuel A Kerk; Alexander Ky; Anne P Ehlers; Oliver A Varban; Costas A Lyssiotis; Carey N Lumeng; Paul M Stemmer; Robert W O'Rourke Journal: Sci Rep Date: 2021-08-30 Impact factor: 4.379