| Literature DB >> 29136915 |
Mihaela Savin1, Carmen-Marinela Mihailescu2, Iulia Matei3, Dana Stan4, Carmen Aura Moldovan5, Marian Ion5, Ion Baciu6.
Abstract
We describe the preparation and validation of a novel lateral flow immunoassay test for the detection of human heart fatty acid binding protein (hFABP). Water-soluble CdTe quantum dots (QDs) were selected as the fluorescent label and were linked covalently to anti-hFABP antibodies. Upon conjugation, the secondary structure of the anti-hFABP was preserved and the fluorescence quantum yield of the CdTe QDs increased. The labelled antibodies were transferred to the immunoassay test strip and the antigen-antibody reaction was successfully performed. This evidenced the preserved antibody activity of QD-labelled anti-hFABP towards hFABP, and provided a rapid means for the quantitation of hFABP in human serum within the range of 0-160ng ∙ ml-1, with a much lower detection limit of 221pg.∙ ml-1 compared with other rapid tests based on lateral flow immunoassays. This new immunoassay test has been successfully used for the early detection of acute myocardial infarction.Entities:
Keywords: Acute myocardial infarction; Fluorescence detection; H-FABP; Lateral flow immuno assay; Quantum dots
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Year: 2017 PMID： 29136915 DOI： 10.1016/j.talanta.2017.10.045
Source DB: PubMed Journal: Talanta ISSN： 0039-9140 Impact factor: 6.057