| Literature DB >> 29134584 |
Abstract
When Bacillus subtilis is infected by bacteriophage SPO1, the phage directs the remodeling of the host cell, converting it into a factory for phage reproduction. Much synthesis of host DNA, RNA, and protein is shut off, and cell division is prevented. Here I describe the protocols by which we have demonstrated those processes, and identified the roles played by specific SPO1 gene products in causing those processes.Entities:
Keywords: Bacillus subtilis; Bacteriophage SPO1; Cell division; Host-shutoff; Host-takeover; Macromolecular syntheses; Resistant mutants
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Year: 2018 PMID： 29134584 DOI： 10.1007/978-1-4939-7343-9_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745