Literature DB >> 29131206

A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex.

Sarah H Hewitt1, Jonathan Parris, Romain Mailhot, Stephen J Butler.   

Abstract

We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.

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Year:  2017        PMID： 29131206     DOI： 10.1039/c7cc05887j

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  11 in total

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Journal:  Cell Chem Biol       Date:  2020-07-30       Impact factor: 8.116

2.  A simple, robust, universal assay for real-time enzyme monitoring by signalling changes in nucleoside phosphate anion concentration using a europium(iii)-based anion receptor.

Authors:  Sarah H Hewitt; Rozee Ali; Romain Mailhot; Chloe R Antonen; Charlotte A Dodson; Stephen J Butler
Journal:  Chem Sci       Date:  2019-05-01       Impact factor: 9.825

3.  HOCl Responsive Lanthanide Complexes Using Hydroquinone Caging Units.

Authors:  Elena Del Giorgio; Thomas Just Sørensen
Journal:  Molecules       Date:  2020-04-23       Impact factor: 4.411

4.  A Quinoline-Appended Cyclodextrin Derivative as a Highly Selective Receptor and Colorimetric Probe for Nucleotides.

Authors:  Kuppusamy Kanagaraj; Chao Xiao; Ming Rao; Chunying Fan; Victor Borovkov; Guo Cheng; Dayang Zhou; Zhihui Zhong; Dan Su; Xingke Yu; Jiabin Yao; Taotao Hao; Wanhua Wu; Jason J Chruma; Cheng Yang
Journal:  iScience       Date:  2020-02-21

5.  Binding and Sensing Properties of a Hybrid Naphthalimide-Pyrene Aza-Cyclophane towards Nucleotides in an Aqueous Solution.

Authors:  Aleksandr M Agafontsev; Aleksandr S Oshchepkov; Tatiana A Shumilova; Evgeny A Kataev
Journal:  Molecules       Date:  2021-02-12       Impact factor: 4.411

6.  Versatile small molecule kinase assay through real-time, ratiometric fluorescence changes based on a pyrene-DPA-Zn2+ complex.

Authors:  Jihoon Kim; Jinyoung Oh; Min Su Han
Journal:  RSC Adv       Date:  2021-03-10       Impact factor: 3.361

7.  Sterically demanding macrocyclic Eu(iii) complexes for selective recognition of phosphate and real-time monitoring of enzymatically generated adenosine monophosphate.

Authors:  Samantha E Bodman; Colum Breen; Sam Kirkland; Simon Wheeler; Erin Robertson; Felix Plasser; Stephen J Butler
Journal:  Chem Sci       Date:  2022-02-11       Impact factor: 9.825

8.  Tuning the anion binding properties of lanthanide receptors to discriminate nucleoside phosphates in a sensing array.

Authors:  Sarah H Hewitt; Georgina Macey; Romain Mailhot; Mark R J Elsegood; Fernanda Duarte; Alan M Kenwright; Stephen J Butler
Journal:  Chem Sci       Date:  2020-03-11       Impact factor: 9.825

9.  Cationic Europium Complexes for Visualizing Fluctuations in Mitochondrial ATP Levels in Living Cells.

Authors:  Romain Mailhot; Thomas Traviss-Pollard; Robert Pal; Stephen J Butler
Journal:  Chemistry       Date:  2018-06-28       Impact factor: 5.236

10.  Anion binding to a cationic europium(III) probe enables the first real-time assay of heparan sulfotransferase activity.

Authors:  Simon Wheeler; Colum Breen; Yong Li; Sarah H Hewitt; Erin Robertson; Edwin A Yates; Igor L Barsukov; David G Fernig; Stephen J Butler
Journal:  Org Biomol Chem       Date:  2022-01-19       Impact factor: 3.876
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