Literature DB >> 29130194

Single mRNA Molecule Detection in Drosophila.

Shawn C Little1, Thomas Gregor2.   

Abstract

Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolute units, enabling new studies of transcriptional mechanisms underlying precision and reproducibility in cell specification.

Entities:  

Keywords:  Drosophila; FISH; Fluorescence imaging; Quantitation; Single molecule

Mesh：

Substances：

Year:  2018        PMID： 29130194      PMCID： PMC5772605          DOI： 10.1007/978-1-4939-7213-5_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  18 in total

1.  Automated detection and quantification of single RNAs at cellular resolution in zebrafish embryos.

Authors:  L Carine Stapel; Benoit Lombardot; Coleman Broaddus; Dagmar Kainmueller; Florian Jug; Eugene W Myers; Nadine L Vastenhouw
Journal:  Development       Date:  2015-12-23       Impact factor: 6.868

2.  Single-Molecule RNA In Situ Hybridization (smFISH) and Immunofluorescence (IF) in the Drosophila Egg Chamber.

Authors:  Livia V Bayer; Mona Batish; Stephen K Formel; Diana P Bratu
Journal:  Methods Mol Biol       Date:  2015

3.  Maternal origins of developmental reproducibility.

Authors:  Mariela D Petkova; Shawn C Little; Feng Liu; Thomas Gregor
Journal:  Curr Biol       Date:  2014-05-22       Impact factor: 10.834

4.  Visualization of single RNA transcripts in situ.

Authors:  A M Femino; F S Fay; K Fogarty; R H Singer
Journal:  Science       Date:  1998-04-24       Impact factor: 47.728

5.  Precise developmental gene expression arises from globally stochastic transcriptional activity.

Authors:  Shawn C Little; Mikhail Tikhonov; Thomas Gregor
Journal:  Cell       Date:  2013-08-15       Impact factor: 41.582

6.  The formation of the Bicoid morphogen gradient requires protein movement from anteriorly localized mRNA.

Authors:  Shawn C Little; Gašper Tkačik; Thomas B Kneeland; Eric F Wieschaus; Thomas Gregor
Journal:  PLoS Biol       Date:  2011-03-01       Impact factor: 8.029

7.  Imaging individual mRNA molecules using multiple singly labeled probes.

Authors:  Arjun Raj; Patrick van den Bogaard; Scott A Rifkin; Alexander van Oudenaarden; Sanjay Tyagi
Journal:  Nat Methods       Date:  2008-09-21       Impact factor: 28.547

8.  Stochastic mRNA synthesis in mammalian cells.

Authors:  Arjun Raj; Charles S Peskin; Daniel Tranchina; Diana Y Vargas; Sanjay Tyagi
Journal:  PLoS Biol       Date:  2006-10       Impact factor: 8.029

9.  Bursty gene expression in the intact mammalian liver.

Authors:  Keren Bahar Halpern; Sivan Tanami; Shanie Landen; Michal Chapal; Liran Szlak; Anat Hutzler; Anna Nizhberg; Shalev Itzkovitz
Journal:  Mol Cell       Date:  2015-02-26       Impact factor: 17.970

10.  Single-cell analysis of transcription kinetics across the cell cycle.

Authors:  Samuel O Skinner; Heng Xu; Sonal Nagarkar-Jaiswal; Pablo R Freire; Thomas P Zwaka; Ido Golding
Journal:  Elife       Date:  2016-01-29       Impact factor: 8.140
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  9 in total

Review 1.  Fishing forward and reverse: Advances in zebrafish phenomics.

Authors:  Ricardo Fuentes; Joaquín Letelier; Benjamin Tajer; Leonardo E Valdivia; Mary C Mullins
Journal:  Mech Dev       Date:  2018-08-18       Impact factor: 1.882

2.  CLAMP regulates zygotic genome activation in Drosophila embryos.

Authors:  Megan M Colonnetta; Juan E Abrahante; Paul Schedl; Daryl M Gohl; Girish Deshpande
Journal:  Genetics       Date:  2021-10-02       Impact factor: 4.402

3.  Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation.

Authors:  Hui Chen; Matthew C Good
Journal:  Methods Enzymol       Date:  2020-03-23       Impact factor: 1.600

4.  Antagonism between germ cell-less and Torso receptor regulates transcriptional quiescence underlying germline/soma distinction.

Authors:  Megan M Colonnetta; Lauren R Lym; Lillian Wilkins; Gretchen Kappes; Elias A Castro; Pearl V Ryder; Paul Schedl; Dorothy A Lerit; Girish Deshpande
Journal:  Elife       Date:  2021-01-18       Impact factor: 8.140

5.  The Wg and Dpp morphogens regulate gene expression by modulating the frequency of transcriptional bursts.

Authors:  Rachael Bakker; Madhav Mani; Richard W Carthew
Journal:  Elife       Date:  2020-06-22       Impact factor: 8.140

6.  A sensitive mNeonGreen reporter system to measure transcriptional dynamics in Drosophila development.

Authors:  Stefano Ceolin; Monika Hanf; Marta Bozek; Andrea Ennio Storti; Nicolas Gompel; Ulrich Unnerstall; Christophe Jung; Ulrike Gaul
Journal:  Commun Biol       Date:  2020-11-12

Review 7.  Fluorescent Platforms for RNA Chemical Biology Research.

Authors:  Jinxi Du; Ricky Dartawan; William Rice; Forrest Gao; Joseph H Zhou; Jia Sheng
Journal:  Genes (Basel)       Date:  2022-07-27       Impact factor: 4.141

8.  CLAMP and Zelda function together to promote Drosophila zygotic genome activation.

Authors:  Jingyue Duan; Leila Rieder; Megan M Colonnetta; Annie Huang; Mary Mckenney; Scott Watters; Girish Deshpande; William Jordan; Nicolas Fawzi; Erica Larschan
Journal:  Elife       Date:  2021-08-03       Impact factor: 8.140

9.  Correct dosage of X chromosome transcription is controlled by a nuclear pore component.

Authors:  Jennifer R Aleman; Terra M Kuhn; Pau Pascual-Garcia; Janko Gospocic; Yemin Lan; Roberto Bonasio; Shawn C Little; Maya Capelson
Journal:  Cell Rep       Date:  2021-06-15       Impact factor: 9.423
  9 in total

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