Yipeng Liu1, Xuan Li2, Chaoqun Ma3, Ping Wang2, Ju Liu4, Hong Su2, Hao Zhuo2, Xianglei Kong2, Dayu Xu5, Dongmei Xu6. 1. Department of Nephrology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, China. Electronic address: liuyipeng@sdhospital.com.cn. 2. Department of Nephrology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, China. 3. Department of Emergency, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, China. 4. Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, China. 5. Department of Nephrology, Zibo Central Hospital, Zibo 255000, China. 6. Department of Nephrology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, China. Electronic address: xudongmei@sdhospital.com.cn.
Abstract
BACKGROUND: The M-type phospholipase A2 receptor (PLA2R) is a specific target autoantigen identified in idiopathic membranous nephropathy (IMN). The autoantibody against PLA2R (anti-PLA2R) may be used to diagnose IMN. However, the appropriate diagnosis cut-off value for Chinese patients with IMN has not been established. METHODS: In total, 119 patients who underwent renal biopsy (57 patients with IMN and 62 patients with non-IMN glomerulonephritis) and 22 healthy individuals were recruited for our observation study from Qianfoshan Hospital between September 2011 and March 2016. The serum concentration of anti-PLA2R was measured using a quantitative enzyme-linked immunosorbent assay (ELISA). The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and receiver operating characteristic (ROC) curve of anti-PLA2R in diagnosing IMN were analysed based on the ELISA detection. RESULTS: The sensitivity, specificity, PPV, and NPV of anti-PLA2R in the diagnosis of IMN in the Chinese patients were 82.5, 75, 69.1, and 86.3% for the 2RU/ml cut-off value; 78.9, 91.7, 86.5, and 86.5% for the 2.6RU/ml cut-off value; 59.6, 95.2, 89.5, and 77.7% for the 14RU/ml cut-off value; 50.9, 96.4, 90.6, and 74.3% for the 20RU/ml cut-off value; and 47.4, 97.6, 93.1, and 73.2% for the 40RU/ml cut-off value, respectively. The area under the ROC curve was 0.879. CONCLUSIONS: The cut-off value of 2.6RU/ml is recommended for the use of anti-PLA2R for the diagnosis of IMN in Chinese patients based on the ELISA.
BACKGROUND: The M-type phospholipase A2 receptor (PLA2R) is a specific target autoantigen identified in idiopathic membranous nephropathy (IMN). The autoantibody against PLA2R (anti-PLA2R) may be used to diagnose IMN. However, the appropriate diagnosis cut-off value for Chinese patients with IMN has not been established. METHODS: In total, 119 patients who underwent renal biopsy (57 patients with IMN and 62 patients with non-IMN glomerulonephritis) and 22 healthy individuals were recruited for our observation study from Qianfoshan Hospital between September 2011 and March 2016. The serum concentration of anti-PLA2R was measured using a quantitative enzyme-linked immunosorbent assay (ELISA). The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and receiver operating characteristic (ROC) curve of anti-PLA2R in diagnosing IMN were analysed based on the ELISA detection. RESULTS: The sensitivity, specificity, PPV, and NPV of anti-PLA2R in the diagnosis of IMN in the Chinese patients were 82.5, 75, 69.1, and 86.3% for the 2RU/ml cut-off value; 78.9, 91.7, 86.5, and 86.5% for the 2.6RU/ml cut-off value; 59.6, 95.2, 89.5, and 77.7% for the 14RU/ml cut-off value; 50.9, 96.4, 90.6, and 74.3% for the 20RU/ml cut-off value; and 47.4, 97.6, 93.1, and 73.2% for the 40RU/ml cut-off value, respectively. The area under the ROC curve was 0.879. CONCLUSIONS: The cut-off value of 2.6RU/ml is recommended for the use of anti-PLA2R for the diagnosis of IMN in Chinese patients based on the ELISA.
Authors: Barbara Seitz-Polski; Karine Dahan; Hanna Debiec; Alexandra Rousseau; Marine Andreani; Christelle Zaghrini; Michel Ticchioni; Alessandra Rosenthal; Sylvia Benzaken; Ghislaine Bernard; Gérard Lambeau; Pierre Ronco; Vincent L M Esnault Journal: Clin J Am Soc Nephrol Date: 2019-07-24 Impact factor: 8.237
Authors: Elion Hoxha; Rolf A K Stahl; Linda Reinhard; Alexander Kühnl; Wolfgang Schlumberger; Cornelia Dähnrich Journal: Kidney Int Rep Date: 2021-01-13
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