| Literature DB >> 29126572 |
Ross A Marklein1, Johnny Lam1, Murat Guvendiren2, Kyung E Sung1, Steven R Bauer3.
Abstract
Heterogeneity in cell function has presented a significant hurdle to the successful clinical translation of many cellular therapies. Current techniques for assessing cell quality and the effects of microenvironmental cues and manufacturing processes on cell behavior often inadequately address heterogeneity due to issues such as population versus single-cell measurements and the therapeutic relevance and throughput/robustness of the assay. Due to the well-established relationship between morphology and cellular function, morphological profiling has become increasingly utilized to better understand functional heterogeneity and its impact on therapeutic development. In this review, we introduce an emerging field we term functionally-relevant morphological profiling with great potential to improve our understanding of cellular heterogeneity through discovering novel quality attributes, optimizing manufacturing, and screening drugs/biomaterials. Published by Elsevier Ltd.Keywords: biomaterials; cellular quality attributes; high-content imaging; high-throughput screening; morphology
Mesh：
Year: 2017 PMID： 29126572 DOI： 10.1016/j.tibtech.2017.10.007
Source DB: PubMed Journal: Trends Biotechnol ISSN： 0167-7799 Impact factor: 19.536