Mohammad Hossein Ahmadi1, Akbar Mirsalehian2, Mohammad Ali Sadighi Gilani3,4, Abbas Bahador1, Malihe Talebi5. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. mirsaleh@tums.ac.ir. 3. Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. ma.sadighi@royaninstitute.org. 4. Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. ma.sadighi@royaninstitute.org. 5. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abstract
OBJECTIVES: To elucidate the association between asymptomatic infections caused by Mycoplasma genitalium and male infertility, and evaluate the role of antibiotic therapy in treatment of this failure. METHODS: A total of 165 infertile males having abnormal semen parameters (study group) and 165 healthy fertile men (control group) were included. Semen samples were taken from all participants and after analyzing for semen parameters, undergone real-time PCR, microbial culture, and reactive oxygen species (ROS), as well as total antioxidant capacity (TAC) assays. Infected individuals of study group were treated with antibiotic. One month after the treatment completion, second semen samples were taken and subjected to all the tests mentioned. The data were analyzed using SPSS statistical software, version 22.0. RESULTS: The frequency of M. genitalium was significantly higher in the infertile men compared with the fertile ones (9.7% vs. 1.2%; p = 0.001). Mean cycle threshold (C t) value was lower in infected infertile than infected fertile men (p < 0.001). All semen parameters, except volume, pH, and viscosity, were improved (p < 0.05), most of which reached their normal range; leukocytes in seminal fluid decreased (p = 0.02), the level of TAC was elevated (p = 0.002), and ROS level as well as ROS/TAC ratio reduced after antibiotic treatment (p = 0.03). Wives of seven infected infertile men (43.8%) became pregnant 4 months after the treatment completion. CONCLUSIONS: Asymptomatic infection caused by M. genitalium is correlated with male infertility and antibiotic therapy can improve the semen quality and be used to treat male infertility.
OBJECTIVES: To elucidate the association between asymptomatic infections caused by Mycoplasma genitalium and male infertility, and evaluate the role of antibiotic therapy in treatment of this failure. METHODS: A total of 165 infertile males having abnormal semen parameters (study group) and 165 healthy fertile men (control group) were included. Semen samples were taken from all participants and after analyzing for semen parameters, undergone real-time PCR, microbial culture, and reactive oxygen species (ROS), as well as total antioxidant capacity (TAC) assays. Infected individuals of study group were treated with antibiotic. One month after the treatment completion, second semen samples were taken and subjected to all the tests mentioned. The data were analyzed using SPSS statistical software, version 22.0. RESULTS: The frequency of M. genitalium was significantly higher in the infertile men compared with the fertile ones (9.7% vs. 1.2%; p = 0.001). Mean cycle threshold (C t) value was lower in infected infertile than infected fertilemen (p < 0.001). All semen parameters, except volume, pH, and viscosity, were improved (p < 0.05), most of which reached their normal range; leukocytes in seminal fluid decreased (p = 0.02), the level of TAC was elevated (p = 0.002), and ROS level as well as ROS/TAC ratio reduced after antibiotic treatment (p = 0.03). Wives of seven infected infertilemen (43.8%) became pregnant 4 months after the treatment completion. CONCLUSIONS: Asymptomatic infection caused by M. genitalium is correlated with male infertility and antibiotic therapy can improve the semen quality and be used to treat male infertility.
Authors: Fabrícia Gimenes; Raquel P Souza; Jaqueline C Bento; Jorge J V Teixeira; Silvya S Maria-Engler; Marcelo G Bonini; Marcia E L Consolaro Journal: Nat Rev Urol Date: 2014-10-21 Impact factor: 14.432
Authors: Lisa E Manhart; Jørgen Skov Jensen; Catriona S Bradshaw; Matthew R Golden; David H Martin Journal: Clin Infect Dis Date: 2015-12-15 Impact factor: 9.079
Authors: Pam Sonnenberg; Catherine A Ison; Soazig Clifton; Nigel Field; Clare Tanton; Kate Soldan; Simon Beddows; Sarah Alexander; Rumena Khanom; Pamela Saunders; Andrew J Copas; Kaye Wellings; Catherine H Mercer; Anne M Johnson Journal: Int J Epidemiol Date: 2015-12 Impact factor: 7.196
Authors: Eugenio Ventimiglia; Paolo Capogrosso; Luca Boeri; Walter Cazzaniga; Rayan Matloob; Edoardo Pozzi; Francesco Chierigo; Costantino Abbate; Paola Viganò; Francesco Montorsi; Andrea Salonia Journal: Hum Reprod Open Date: 2020-09-22
Authors: Somadina I Okwelogu; Joseph I Ikechebelu; Nneka R Agbakoba; Kingsley C Anukam Journal: Front Cell Infect Microbiol Date: 2021-10-01 Impact factor: 5.293
Authors: Emilia Morawiec; Michał Czerwiński; Anna Bednarska- Czerwińska; Andrzej Wiczkowski Journal: Front Cell Infect Microbiol Date: 2022-09-13 Impact factor: 6.073