Αikaterini K Chalkia1,2, Stavros Derdas2,3, Georgios Bontzos1,2, George Sourvinos2,3, Εfstathios T Detorakis4,5. 1. Department of Ophthalmology, University General Hospital of Heraklion, Crete, Greece. 2. Faculty of Medicine, University of Crete, Heraklion, Crete, Greece. 3. Department of Clinical Virology, School of Medicine, University of Crete Heraklion, Crete, Greece. 4. Department of Ophthalmology, University General Hospital of Heraklion, Crete, Greece. detorakis@hotmail.com. 5. Faculty of Medicine, University of Crete, Heraklion, Crete, Greece. detorakis@hotmail.com.
Abstract
PURPOSE: The evaluation of a non-invasive detection method for human papilloma virus (HPV) in ophthalmic pterygia. METHODS: Cotton swab samples and corresponding tissue specimens were collected from 21 ophthalmic pterygia of 21 patients. HPV detection and typing were performed by real-time PCR. Discrepancies in HPV detection between swab and tissue samples as well as clinical correlations of findings were examined. RESULTS: HPV DNA was detected in 9 (42.86%) tissue specimens and 8 (38.09%) respective swab specimens. HPV genotypes 33, 39, 45, 56, 59 and 66 were identified in the examined specimens, while more than one strain's HPV type was detected in 2 specimens. HPV presence was significantly correlated with the female gender whereas other clinical associations were not statistically significant. CONCLUSIONS: Findings imply that PCR-mediated HPV detection and typing in exfoliative swab specimens may be employed as a non-invasive diagnostic tool in the management of ophthalmic pterygia.
PURPOSE: The evaluation of a non-invasive detection method for human papilloma virus (HPV) in ophthalmic pterygia. METHODS: Cotton swab samples and corresponding tissue specimens were collected from 21 ophthalmic pterygia of 21 patients. HPV detection and typing were performed by real-time PCR. Discrepancies in HPV detection between swab and tissue samples as well as clinical correlations of findings were examined. RESULTS:HPV DNA was detected in 9 (42.86%) tissue specimens and 8 (38.09%) respective swab specimens. HPV genotypes 33, 39, 45, 56, 59 and 66 were identified in the examined specimens, while more than one strain's HPV type was detected in 2 specimens. HPV presence was significantly correlated with the female gender whereas other clinical associations were not statistically significant. CONCLUSIONS: Findings imply that PCR-mediated HPV detection and typing in exfoliative swab specimens may be employed as a non-invasive diagnostic tool in the management of ophthalmic pterygia.
Authors: Elmar A Joura; Anna R Giuliano; Ole-Erik Iversen; Celine Bouchard; Constance Mao; Jesper Mehlsen; Edson D Moreira; Yuen Ngan; Lone Kjeld Petersen; Eduardo Lazcano-Ponce; Punnee Pitisuttithum; Jaime Alberto Restrepo; Gavin Stuart; Linn Woelber; Yuh Cheng Yang; Jack Cuzick; Suzanne M Garland; Warner Huh; Susanne K Kjaer; Oliver M Bautista; Ivan S F Chan; Joshua Chen; Richard Gesser; Erin Moeller; Michael Ritter; Scott Vuocolo; Alain Luxembourg Journal: N Engl J Med Date: 2015-02-19 Impact factor: 91.245