Alexandre Cc Vieira1, Joana Magalhães1, Sónia Rocha2, Marcos S Cardoso2, Susana G Santos3, Margarida Borges2, Marina Pinheiro1, Salette Reis1. 1. UCIBIO, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal. 2. UCIBIO, REQUIMTE, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Portugal. 3. INEB - Instituto de Engenharia Biomédica, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal.
Abstract
AIM: This work aims to develop a mannosylated nanostructured lipid carrier (NLC) loaded with rifampicin to improve tuberculosis treatment. MATERIALS & METHODS: An active targeting strategy was used and the nanoparticles were characterized. Effects on cell viability and the antimycobacterial activity of the nanoformulations were evaluated. RESULTS: The nanoparticles developed exhibited a size of about 315 nm and polydispersity <0.2. The drug encapsulation efficiency was higher than 90% and its release was sensitive to pH. The mannosylated NLCs showed efficient uptake by bone marrow derived macrophages. Further, rifampicin-loaded mannosylated NLCs were more efficient in inducing a decrease of intracellular growth of mycobacteria. CONCLUSION: The NLCs developed can be used as a promising carrier for safer and efficient management of tuberculosis.
AIM: This work aims to develop a mannosylated nanostructured lipid carrier (NLC) loaded with rifampicin to improve tuberculosis treatment. MATERIALS & METHODS: An active targeting strategy was used and the nanoparticles were characterized. Effects on cell viability and the antimycobacterial activity of the nanoformulations were evaluated. RESULTS: The nanoparticles developed exhibited a size of about 315 nm and polydispersity <0.2. The drug encapsulation efficiency was higher than 90% and its release was sensitive to pH. The mannosylated NLCs showed efficient uptake by bone marrow derived macrophages. Further, rifampicin-loaded mannosylated NLCs were more efficient in inducing a decrease of intracellular growth of mycobacteria. CONCLUSION: The NLCs developed can be used as a promising carrier for safer and efficient management of tuberculosis.
Entities:
Keywords:
Mycobacterium tuberculosis; active targeting; drug delivery; infectious diseases; nanomedicine
Authors: Aristote B Buya; Bwalya A Witika; Alain M Bapolisi; Chiluba Mwila; Grady K Mukubwa; Patrick B Memvanga; Pedzisai A Makoni; Christian I Nkanga Journal: Pharmaceutics Date: 2021-11-30 Impact factor: 6.321