Yue Wang1, Wei Xu1, Zexi Chen1,2, Bing Han1,2, Mohammad E Haque1,2, Aizhong Liu3. 1. Yunnan Key Laboratory for Wild Plant Resources, Department of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China. 2. University of Chinese Academy of Sciences, Beijing, China. 3. Yunnan Key Laboratory for Wild Plant Resources, Department of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China. liuaizhong@mail.kib.ac.cn.
Abstract
MAIN CONCLUSION: Nuclear Factor-Y transcription factors, which function in regulating seed development (including storage reservoir accumulation) and responding to abiotic stresses, were identified and characterized in castor bean. Nuclear Factor-Y (NF-Y) transcription factors in plants contain three subunits (NF-YA, NF-YB and NF-YC), and function as a heterodimer or heterotrimer complex in regulating plant growth, development and response to stresses. Castor bean (Ricinus communis, Euphorbiaceae) one of the most economically important non-edible oilseed crops, able to grow in diverse soil conditions and displays high tolerance to abiotic stresses. Due to increasing demands for its seed oils, it is necessary to elucidate the molecular mechanism underlying the regulation of growth and development. Based on the available genome data, we identified 25 RcNF-Y members including six RcNF-YAs, 12 RcNF-YBs and seven RcNF-YCs, and characterized their gene structures. Yeast two-hybrid assays confirmed the protein-protein interactions among three subunits. Using transcriptomic data from different tissues, we found that six members were highly or specifically expressed in endosperms (in particular, two LEC1-type members RcNF-YB2 and RcNF-YB12), implying their involvement in regulating seed development and storage reservoir accumulation. Further, we investigated the expression changes of RcNF-Y members in two-week-old seedlings under drought, cold, hot and salt stresses. We found that the expression levels of 20 RcNF-Y members tested were changed and three RcNF-Y members might function in response to abiotic stresses. This study is the first reported on genomic characterization of NF-Y transcription factors in the family Euphorbiaceae. Our results provide the basis for improved understanding of how NF-Y genes function in the regulation of seed development and responses to abiotic stresses in both castor bean and other plants in this family.
MAIN CONCLUSION: Nuclear Factor-Y transcription factors, which function in regulating seed development (including storage reservoir accumulation) and responding to abiotic stresses, were identified and characterized in castor bean. Nuclear Factor-Y (NF-Y) transcription factors in plants contain three subunits (NF-YA, NF-YB and NF-YC), and function as a heterodimer or heterotrimer complex in regulating plant growth, development and response to stresses. Castor bean (Ricinus communis, Euphorbiaceae) one of the most economically important non-edible oilseed crops, able to grow in diverse soil conditions and displays high tolerance to abiotic stresses. Due to increasing demands for its seed oils, it is necessary to elucidate the molecular mechanism underlying the regulation of growth and development. Based on the available genome data, we identified 25 RcNF-Y members including six RcNF-YAs, 12 RcNF-YBs and seven RcNF-YCs, and characterized their gene structures. Yeast two-hybrid assays confirmed the protein-protein interactions among three subunits. Using transcriptomic data from different tissues, we found that six members were highly or specifically expressed in endosperms (in particular, two LEC1-type members RcNF-YB2 and RcNF-YB12), implying their involvement in regulating seed development and storage reservoir accumulation. Further, we investigated the expression changes of RcNF-Y members in two-week-old seedlings under drought, cold, hot and salt stresses. We found that the expression levels of 20 RcNF-Y members tested were changed and three RcNF-Y members might function in response to abiotic stresses. This study is the first reported on genomic characterization of NF-Y transcription factors in the family Euphorbiaceae. Our results provide the basis for improved understanding of how NF-Y genes function in the regulation of seed development and responses to abiotic stresses in both castor bean and other plants in this family.
Authors: T Lotan; M Ohto; K M Yee; M A West; R Lo; R W Kwong; K Yamagishi; R L Fischer; R B Goldberg; J J Harada Journal: Cell Date: 1998-06-26 Impact factor: 41.582
Authors: Nicholas Siefers; Kristen K Dang; Roderick W Kumimoto; William Edwards Bynum; Gregory Tayrose; Ben F Holt Journal: Plant Physiol Date: 2008-11-19 Impact factor: 8.340
Authors: Alexandro Cagliari; Andreia Carina Turchetto-Zolet; Ana Paula Korbes; Felipe Dos Santos Maraschin; Rogerio Margis; Marcia Margis-Pinheiro Journal: Genomics Date: 2014-04-02 Impact factor: 5.736
Authors: Kishor Prabhakar Panzade; Sonam S Kale; M L Manoj; Sonali P Kothawale; Dipak N Damse Journal: Appl Biochem Biotechnol Date: 2021-11-03 Impact factor: 2.926
Authors: P Maheshwari; Divya Kummari; Sudhakar Reddy Palakolanu; U Nagasai Tejaswi; M Nagaraju; G Rajasheker; G Jawahar; N Jalaja; P Rathnagiri; P B Kavi Kishor Journal: PLoS One Date: 2019-09-19 Impact factor: 3.240
Authors: Juan Li; Kai Gao; Xiaoyu Yang; Bin Guo; Yinxuan Xue; Deyu Miao; Sai Huang; Xinmin An Journal: Int J Mol Sci Date: 2022-03-14 Impact factor: 5.923