Fernanda Pessanha de Oliveira1, Bruna Maiara Ferreira Barreto Pires, Keila de Cássia Ferreira de Almeida Silva, Bernadete Teixeira Ferreira de Carvalho, Lenise Arneiro Teixeira, Geraldo Renato de Paula, Beatriz Guitton Renaud Baptista de Oliveira. 1. Fernanda Pessanha de Oliveira, MSN, RN, Nursing School Aurora de Afonso Costa, Universidade Federal Fluminense, Niterói, Brazil. Bruna Maiara Ferreira Barreto Pires, MSN, RN, Nursing School Aurora de Afonso Costa, Universidade Federal Fluminense, Niterói, Brazil. Keila de Cássia Ferreira de Almeida Silva, MPH, BP, Faculty of Pharmacy, Universidade Federal Fluminense, Niterói, Brazil. Bernadete Teixeira Ferreira de Carvalho, DSC, Institute of Microbiology Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil. Lenise Arneiro Teixeira, DSC, BP, Faculty of Pharmacy, Universidade Federal Fluminense, Niterói, Brazil. Geraldo Renato de Paula, DSC, BP, Faculty of Pharmacy, Universidade Federal Fluminense, Niterói, Brazil. Beatriz Guitton Renaud Baptista de Oliveira, DN, RN, Nursing School Aurora de Afonso Costa, Universidade Federal Fluminense, Niterói, Brazil.
Abstract
PURPOSE: Our purposes in this study were to (1) identify Pseudomonas aeruginosa strains collected from swabs of chronic wounds, (2) evaluate the susceptibility of P. aeruginosa strains to various antimicrobials, (3) detect the presence of virulence factors exoenzyme S (exoS) and exoenzyme U (exoU) in P. aeruginosa strains, and (4) evaluate wound colonization by P. aeruginosa via pulsed-field gel electrophoresis (PFGE). DESIGN: Descriptive research using a quantitative approach. SAMPLE AND SETTING: Swabs from 43 adults with chronic wounds treated in an outpatient setting in Niterói City, Brazil, were included using convenience sampling. METHODS: Swabs were collected at 2 points during treatment, 30 to 45 days apart. P. aeruginosa isolates were identified using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. Antimicrobial susceptibility testing was performed using the disk diffusion method. The presence of exoS and exoU genes was evaluated using polymerase chain reaction. Genotyping diversity was determined through PFGE. RESULTS: Forty-eight P. aeruginosa isolates were detected in chronic wounds, and 3 were multidrug resistant (6%). Resistance to aztreonam and ciprofloxacin was observed in 48% and 27% of isolates, respectively. The presence of the exoS gene was verified in 54% of isolates, and 27% were positive for the exoU gene. In most wounds, P. aeruginosa strains had the same genetic characteristics at the 2 time points analyzed, indicating that the wound beds remained colonized. CONCLUSIONS: P. aeruginosa was present in 75% of tested chronic wound samples, and the same clones persisted for more than 1 month. In addition, most bacteria contained virulence genes that were associated with high potential to establish infection. The use of silver in chronic wounds may be associated with multidrug resistance in P. aeruginosa; therefore, it is important to avoid colonization by these bacteria.
PURPOSE: Our purposes in this study were to (1) identify Pseudomonas aeruginosa strains collected from swabs of chronic wounds, (2) evaluate the susceptibility of P. aeruginosa strains to various antimicrobials, (3) detect the presence of virulence factors exoenzyme S (exoS) and exoenzyme U (exoU) in P. aeruginosa strains, and (4) evaluate wound colonization by P. aeruginosa via pulsed-field gel electrophoresis (PFGE). DESIGN: Descriptive research using a quantitative approach. SAMPLE AND SETTING: Swabs from 43 adults with chronic wounds treated in an outpatient setting in Niterói City, Brazil, were included using convenience sampling. METHODS: Swabs were collected at 2 points during treatment, 30 to 45 days apart. P. aeruginosa isolates were identified using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. Antimicrobial susceptibility testing was performed using the disk diffusion method. The presence of exoS and exoU genes was evaluated using polymerase chain reaction. Genotyping diversity was determined through PFGE. RESULTS: Forty-eight P. aeruginosa isolates were detected in chronic wounds, and 3 were multidrug resistant (6%). Resistance to aztreonam and ciprofloxacin was observed in 48% and 27% of isolates, respectively. The presence of the exoS gene was verified in 54% of isolates, and 27% were positive for the exoU gene. In most wounds, P. aeruginosa strains had the same genetic characteristics at the 2 time points analyzed, indicating that the wound beds remained colonized. CONCLUSIONS:P. aeruginosa was present in 75% of tested chronic wound samples, and the same clones persisted for more than 1 month. In addition, most bacteria contained virulence genes that were associated with high potential to establish infection. The use of silver in chronic wounds may be associated with multidrug resistance in P. aeruginosa; therefore, it is important to avoid colonization by these bacteria.
Authors: Beatriz G R B de Oliveira; Fernanda P de Oliveira; Lenise A Teixeira; Geraldo R de Paula; Bianca C de Oliveira; Bruna M F B Pires Journal: Int Wound J Date: 2019-10-01 Impact factor: 3.315
Authors: S Lee McGill; Yeni Yung; Kristopher A Hunt; Michael A Henson; Luke Hanley; Ross P Carlson Journal: Sci Rep Date: 2021-01-14 Impact factor: 4.379
Authors: Anne S Laulund; Franziska Schwartz; Hannah Trøstrup; Kim Thomsen; Lars Christophersen; Henrik Calum; Oana Ciofu; Niels Høiby; Claus Moser Journal: Front Cell Infect Microbiol Date: 2021-04-12 Impact factor: 5.293