Literature DB >> 29116510

Parallelized Antibody Selection in Microtiter Plates.

Giulio Russo1, Doris Meier1, Saskia Helmsing1, Esther Wenzel1, Fabian Oberle1, André Frenzel2,3, Michael Hust4.   

Abstract

The most common in vitro technology to generate human antibodies is phage display. This technology is a key technology to select recombinant antibodies for the use as research tools, in diagnostic tests, and for the development of therapeutics.In this review, the high-throughput compatible selection of antibodies (scFv) in microtiter plates is described. The given detailed protocols allow the antibody selection ("panning"), screening and identification of monoclonal antibodies in less than 1 week.

Entities:  

Keywords:  Antibody; Antibody selection; Monoclonal antibody screening; Panning; Phage display; Single-chain fragment variable (scFv)

Mesh：

Substances：

Year:  2018        PMID： 29116510     DOI： 10.1007/978-1-4939-7447-4_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

Review 1.  Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy.

Authors:  Kristian Daniel Ralph Roth; Esther Veronika Wenzel; Maximilian Ruschig; Stephan Steinke; Nora Langreder; Philip Alexander Heine; Kai-Thomas Schneider; Rico Ballmann; Viola Fühner; Philipp Kuhn; Thomas Schirrmann; André Frenzel; Stefan Dübel; Maren Schubert; Gustavo Marçal Schmidt Garcia Moreira; Federico Bertoglio; Giulio Russo; Michael Hust
Journal:  Front Cell Infect Microbiol       Date:  2021-07-07       Impact factor: 5.293

2.  The Conserved Cys-2232 in Clostridioides difficile Toxin B Modulates Receptor Binding.

Authors:  Soo-Young Chung; Dennis Schöttelndreier; Helma Tatge; Viola Fühner; Michael Hust; Lara-Antonia Beer; Ralf Gerhard
Journal:  Front Microbiol       Date:  2018-10-26       Impact factor: 5.640

3.  Pyruvate dehydrogenase complex-enzyme 2, a new target for Listeria spp. detection identified using combined phage display technologies.

Authors:  Gustavo Marçal Schmidt Garcia Moreira; Sarah Mara Stella Köllner; Saskia Helmsing; Lothar Jänsch; Anja Meier; Sabine Gronow; Christian Boedeker; Stefan Dübel; Marcelo Mendonça; Ângela Nunes Moreira; Fabricio Rochedo Conceição; Michael Hust
Journal:  Sci Rep       Date:  2020-09-17       Impact factor: 4.379

4.  SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface.

Authors:  Federico Bertoglio; Doris Meier; Nora Langreder; Stephan Steinke; Ulfert Rand; Luca Simonelli; Philip Alexander Heine; Rico Ballmann; Kai-Thomas Schneider; Kristian Daniel Ralph Roth; Maximilian Ruschig; Peggy Riese; Kathrin Eschke; Yeonsu Kim; Dorina Schäckermann; Mattia Pedotti; Philipp Kuhn; Susanne Zock-Emmenthal; Johannes Wöhrle; Normann Kilb; Tobias Herz; Marlies Becker; Martina Grasshoff; Esther Veronika Wenzel; Giulio Russo; Andrea Kröger; Linda Brunotte; Stephan Ludwig; Viola Fühner; Stefan Daniel Krämer; Stefan Dübel; Luca Varani; Günter Roth; Luka Čičin-Šain; Maren Schubert; Michael Hust
Journal:  Nat Commun       Date:  2021-03-11       Impact factor: 14.919

5.  Phage Display-Derived Monoclonal Antibodies Against Internalins A and B Allow Specific Detection of Listeria monocytogenes.

Authors:  Gustavo Marçal Schmidt Garcia Moreira; Sabine Gronow; Stefan Dübel; Marcelo Mendonça; Ângela Nunes Moreira; Fabricio Rochedo Conceição; Michael Hust
Journal:  Front Public Health       Date:  2022-03-15

Review 6.  Single-Chain Fragment Variable: Recent Progress in Cancer Diagnosis and Therapy.

Authors:  Paola Muñoz-López; Rosa María Ribas-Aparicio; Elayne Irene Becerra-Báez; Karla Fraga-Pérez; Luis Fernando Flores-Martínez; Armando Alfredo Mateos-Chávez; Rosendo Luria-Pérez
Journal:  Cancers (Basel)       Date:  2022-08-30       Impact factor: 6.575

7.  A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations.

Authors:  Federico Bertoglio; Viola Fühner; Maximilian Ruschig; Philip Alexander Heine; Leila Abassi; Thomas Klünemann; Ulfert Rand; Doris Meier; Nora Langreder; Stephan Steinke; Rico Ballmann; Kai-Thomas Schneider; Kristian Daniel Ralph Roth; Philipp Kuhn; Peggy Riese; Dorina Schäckermann; Janin Korn; Allan Koch; M Zeeshan Chaudhry; Kathrin Eschke; Yeonsu Kim; Susanne Zock-Emmenthal; Marlies Becker; Margitta Scholz; Gustavo Marçal Schmidt Garcia Moreira; Esther Veronika Wenzel; Giulio Russo; Hendrikus S P Garritsen; Sebastian Casu; Andreas Gerstner; Günter Roth; Julia Adler; Jakob Trimpert; Andreas Hermann; Thomas Schirrmann; Stefan Dübel; André Frenzel; Joop Van den Heuvel; Luka Čičin-Šain; Maren Schubert; Michael Hust
Journal:  Cell Rep       Date:  2021-07-07       Impact factor: 9.995

8.  The Binary Toxin CDT of Clostridium difficile as a Tool for Intracellular Delivery of Bacterial Glucosyltransferase Domains.

Authors:  Lara-Antonia Beer; Helma Tatge; Carmen Schneider; Maximilian Ruschig; Michael Hust; Jessica Barton; Stefan Thiemann; Viola Fühner; Giulio Russo; Ralf Gerhard
Journal:  Toxins (Basel)       Date:  2018-06-01       Impact factor: 4.546
  8 in total

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