Simeon Eche1, Irene Mackraj1, Jagidesa Moodley2. 1. a Discipline of Human Physiology , University of KwaZulu-Natal , Durban , South Africa. 2. b Discipline of Obstetrics and Gynecology and Women's Health and HIV Research Group , Nelson R Mandela School of Medicine, University of KwaZulu-Natal , Durban , South Africa.
Abstract
OBJECTIVE: Quantification of circulating fetal and total cell-free DNA (cfDNA) and soluble human leucocyte antigen (HLAG) in gestational hypertension and pre-eclampsia. METHODS: Serum cfDNA were quantified in controls, pre-eclamptics, and gestational hypertensive patients using real-time qPCR. Soluble HLAG was measured by enzyme-linked immune-sorbent assay. RESULTS: Serum fetal and total cfDNA levels were higher in pre-eclampsia compared with the controls and gestational hypertensives (p < 0.001), more so in severe compared with mild-to-moderate pre-eclampsia (p < 0.05). Soluble HLAG levels were lower in pre-eclamptics than controls and gestational hypertension (p < 0.05). CONCLUSION: Circulating fetal and total cfDNA were increased, while soluble HLAG was decreased in pre-eclampsia.
OBJECTIVE: Quantification of circulating fetal and total cell-free DNA (cfDNA) and soluble human leucocyte antigen (HLAG) in gestational hypertension and pre-eclampsia. METHODS: Serum cfDNA were quantified in controls, pre-eclamptics, and gestational hypertensivepatients using real-time qPCR. Soluble HLAG was measured by enzyme-linked immune-sorbent assay. RESULTS: Serum fetal and total cfDNA levels were higher in pre-eclampsia compared with the controls and gestational hypertensives (p < 0.001), more so in severe compared with mild-to-moderate pre-eclampsia (p < 0.05). Soluble HLAG levels were lower in pre-eclamptics than controls and gestational hypertension (p < 0.05). CONCLUSION: Circulating fetal and total cfDNA were increased, while soluble HLAG was decreased in pre-eclampsia.
Entities:
Keywords:
Fetal cell-free DNA; gestational hypertension; pre-eclampsia; soluble human leucocyte antigen; total cell-free DNA
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