| Literature DB >> 29114059 |
Fabio M Gomes1, Bretta L Hixson1, Miles D W Tyner1, Jose Luis Ramirez1, Gaspar E Canepa1, Thiago Luiz Alves E Silva1, Alvaro Molina-Cruz1, Moussa Keita2, Fouseyni Kane2, Boïssé Traoré2, Nafomon Sogoba2, Carolina Barillas-Mury3.
Abstract
A naturally occurring Wolbachia strain (wAnga-Mali) was identified in mosquitoes of the Anopheles gambiae complex collected in the Malian villages of Dangassa and Kenieroba. Phylogenetic analysis of the nucleotide sequence of two 16S rRNA regions showed that wAnga-Mali clusters with Wolbachia strains from supergroup A and has the highest homology to a Wolbachia strain isolated from cat fleas (Ctenocephalides). wAnga-Mali is different from two Wolbachia strains previously reported in A. gambiae from Burkina Faso (wAnga_VK5_STP and wAnga_VK5_3.1a). Quantitative analysis of Wolbachia and Plasmodium sporozoite infection in field-collected mosquitoes indicates that the prevalence and intensity of Plasmodium falciparum sporozoite infection is significantly lower in Wolbachia-infected females. The presence of Wolbachia in females from a laboratory Anopheles coluzzii (A. gambiae, M form) colony experimentally infected with P. falciparum (NF54 strain) gametocyte cultures slightly enhanced oocyst infection. However, Wolbachia infection significantly reduced the prevalence and intensity of sporozoite infection, as observed in the field. This indicates that wAnga-Mali infection does not limit early stages of Plasmodium infection in the mosquito, but it has a strong deleterious effect on sporozoites and reduces malaria transmission.Entities:
Keywords: Anopheles; Plasmodium; Wolbachia; malaria; mosquito
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Year: 2017 PMID： 29114059 PMCID： PMC5703331 DOI： 10.1073/pnas.1716181114
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205