Yanliang Li1, Xiaowen Wen2, Longgang Wang2, Xianjun Sun2, Heng Ma2, Zheng Fu3, Leping Li4. 1. Department of General Surgery, Shandong Cancer Hospital Affiliated to Shandong University, Shandong Academy of Medical Sciences, NO.440 Jiyan Road, Jinan City 250117, Shandong Province, PR China; Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, NO.324 Jingwuweiqi Road, Jinan City 250021, Shandong Province, PR China. 2. Department of General Surgery, Shandong Cancer Hospital Affiliated to Shandong University, Shandong Academy of Medical Sciences, NO.440 Jiyan Road, Jinan City 250117, Shandong Province, PR China. 3. Department of Radiology, Shandong Cancer Hospital Affiliated to Shandong University, Shandong Academy of Medical Sciences, NO.440 Jiyan Road, Jinan City 250117, Shandong Province, PR China. Electronic address: moon_edu@163.com. 4. Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, NO.324 Jingwuweiqi Road, Jinan City 250021, Shandong Province, PR China. Electronic address: lileping@medmail.com.cn.
Abstract
OBJECTIVES: LncRNA ZEB1 Antisense 1 (ZEB1-AS1) has been suggested to be an oncogenic role in human hepatocellular carcinoma, osteosarcoma, glioma and esophageal carcinoma progression. However, the clinical significance and biological function of ZEB1-AS1 in gastric cancer is poorly understood. METHODS: Levels of ZEB1-AS1 expression in gastric cancer tissues and cell lines were detected by qRT-PCR. Loss-of-function and gain-of-function studies were conducted to explore the biological function of ZEB1-AS1 in gastric cancer cells migration, invasion and EMT process. Rescued-function studies were performed to explore the association between ZEB1-AS1 and ZEB1 in gastric cancer cells migration, invasion and EMT process. RESULTS: ZEB1-AS1 was overexpressed in gastric cancer tissues and cell lines, and correlated with malignant status and prognosis in gastric cancer patients. ZEB1-AS1 regulated gastric cancer cells migration, invasion and EMT process. ZEB1-AS1 positively regulated ZEB1 expression in gastric cancer cells, and had a strongly positive correlation with ZEB1 expression in gastric cancer tissues. Rescued-function studies showed ZEB1 was critical for ZEB1-AS1 induced gastric cancer cells migration, invasion and EMT process. CONCLUSIONS: ZEB1-AS1 served as oncogenic roles in the regulation of gastric cancer cells migration, invasion and EMT process through modulating ZEB1.
OBJECTIVES: LncRNA ZEB1 Antisense 1 (ZEB1-AS1) has been suggested to be an oncogenic role in humanhepatocellular carcinoma, osteosarcoma, glioma and esophageal carcinoma progression. However, the clinical significance and biological function of ZEB1-AS1 in gastric cancer is poorly understood. METHODS: Levels of ZEB1-AS1 expression in gastric cancer tissues and cell lines were detected by qRT-PCR. Loss-of-function and gain-of-function studies were conducted to explore the biological function of ZEB1-AS1 in gastric cancer cells migration, invasion and EMT process. Rescued-function studies were performed to explore the association between ZEB1-AS1 and ZEB1 in gastric cancer cells migration, invasion and EMT process. RESULTS:ZEB1-AS1 was overexpressed in gastric cancer tissues and cell lines, and correlated with malignant status and prognosis in gastric cancerpatients. ZEB1-AS1 regulated gastric cancer cells migration, invasion and EMT process. ZEB1-AS1 positively regulated ZEB1 expression in gastric cancer cells, and had a strongly positive correlation with ZEB1 expression in gastric cancer tissues. Rescued-function studies showed ZEB1 was critical for ZEB1-AS1 induced gastric cancer cells migration, invasion and EMT process. CONCLUSIONS:ZEB1-AS1 served as oncogenic roles in the regulation of gastric cancer cells migration, invasion and EMT process through modulating ZEB1.
Authors: Narasimha M Beeraka; Hao Gu; Nannan Xue; Yang Liu; Huiming Yu; Junqi Liu; Kuo Chen; Vladimir N Nikolenko; Ruitai Fan Journal: Exp Biol Med (Maywood) Date: 2022-01-22