Anders Abildgaard1,2, Betina Elfving1, Marianne Hokland3, Gregers Wegener1,4, Sten Lund5. 1. a Translational Neuropsychiatry Unit , Aarhus University , Risskov , Denmark. 2. b Department of Clinical Biochemistry , Aarhus University Hospital , Aarhus , Denmark. 3. c Department of Biomedicine , Aarhus University , Aarhus , Denmark. 4. d Center of Excellence for Pharmaceutical Sciences , North-West University (Potchefstroom Campus) , Potchefstroom , South Africa. 5. e Department of Medical Endocrinology (MEA) , Aarhus University Hospital , Aarhus , Denmark.
Abstract
CONTEXT: We have previously shown that an antidepressant-like effect of probiotics in rats was associated with a higher plasma level of the microbial tryptophan metabolite indole-3-propionic acid (IPA). OBJECTIVE: We therefore wanted to study the isolated effect of IPA on behaviour and glucose metabolism in rats. METHODS: Male Sprague-Dawley rats were fed control or IPA-enriched diet for six weeks (n = 12 per group) and assessed in the elevated plus maze, open field and forced swim test. Blood glucose, metabolic hormones and the white blood cell (WBC) composition were analysed. RESULTS: IPA (mean intake 27.3 mg/kg/day) significantly lowered fasting blood glucose level by 0.42 mM (95% CI 0.11-0.73). Similarly, fasting plasma insulin levels and the homeostatic model assessment (HOMA) index of insulin resistance were reduced, whereas plasma metabolic hormones, behaviour and WBC composition remained unaffected by IPA. CONCLUSIONS: Our findings highlight IPA as a promising candidate for treatment of metabolic disorders associated with insulin resistance.
CONTEXT: We have previously shown that an antidepressant-like effect of probiotics in rats was associated with a higher plasma level of the microbial tryptophan metabolite indole-3-propionic acid (IPA). OBJECTIVE: We therefore wanted to study the isolated effect of IPA on behaviour and glucose metabolism in rats. METHODS: Male Sprague-Dawley rats were fed control or IPA-enriched diet for six weeks (n = 12 per group) and assessed in the elevated plus maze, open field and forced swim test. Blood glucose, metabolic hormones and the white blood cell (WBC) composition were analysed. RESULTS:IPA (mean intake 27.3 mg/kg/day) significantly lowered fasting blood glucose level by 0.42 mM (95% CI 0.11-0.73). Similarly, fasting plasma insulin levels and the homeostatic model assessment (HOMA) index of insulin resistance were reduced, whereas plasma metabolic hormones, behaviour and WBC composition remained unaffected by IPA. CONCLUSIONS: Our findings highlight IPA as a promising candidate for treatment of metabolic disorders associated with insulin resistance.
Authors: Dustin M Lee; Kayl E Ecton; S Raj J Trikha; Scott D Wrigley; Keely N Thomas; Micah L Battson; Yuren Wei; Sarah A Johnson; Tiffany L Weir; Christopher L Gentile Journal: Am J Physiol Gastrointest Liver Physiol Date: 2020-05-18 Impact factor: 4.052
Authors: David K Scoville; Cindy Yanfei Li; Dongfang Wang; Joseph L Dempsey; Daniel Raftery; Sridhar Mani; Haiwei Gu; Julia Yue Cui Journal: Drug Metab Dispos Date: 2019-05-23 Impact factor: 3.922