Tereza de Jesus Pinheiro Gomes Bandeira1, Débora de Souza Collares Maia Castelo-Branco2, Marcos Fábio Gadelha Rocha3, Rossana de Aguiar Cordeiro2, Crister José Ocadaque2, Manoel de Araújo Neto Paiva4, Raimunda Sâmia Nogueira Brilhante5, José Júlio Costa Sidrim2. 1. Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil; Christus School of Medicine, Fortaleza, Ceará, Brazil. 2. Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil. 3. Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil; Postgraduate Program in Veterinary Sciences, State University of Ceará, Fortaleza, Ceará, Brazil. 4. Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil; Federal Institute of Education, Science and Technology of Ceará, Acaraú, Ceará, Brazil. 5. Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: brilhante@ufc.br.
Abstract
OBJECTIVE: To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei (B. pseudomallei) recovered in Ceará, Brazil, and screen these isolates for the presence of type three secretion system virulence gene. METHODS: Nineteen B. pseudomallei isolates (9 from clinical cases and 10 from soils) were analyzed. Random amplified polymorphic DNA was performed with primers OPQ-2, OPQ-4 and OPQ-16 to evaluate the genetic diversity, and type three secretion system gene was detected through polymerase chain reaction. RESULTS: Random amplified polymorphic DNA showed a genetic relatedness of approximately 50% among the tested B. pseudomallei isolates, which were grouped into two clades, of which the biggest ones comprised 18/19 isolates for primer OPQ-2, and 17/19 isolates for primer OPQ-16. Primer OPQ-4 grouped the isolates into three clades comprising 1/19, 3/19 and 15/19 isolates. Additionally, type three secretion system gene was detected in all tested isolates. CONCLUSIONS: This is an effort to type B. pseudomallei strains from Ceará, which is important for better understanding this pathogen, contributing for the epidemiological surveillance of melioidosis in this endemic region.
OBJECTIVE: To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei (B. pseudomallei) recovered in Ceará, Brazil, and screen these isolates for the presence of type three secretion system virulence gene. METHODS: Nineteen B. pseudomallei isolates (9 from clinical cases and 10 from soils) were analyzed. Random amplified polymorphic DNA was performed with primers OPQ-2, OPQ-4 and OPQ-16 to evaluate the genetic diversity, and type three secretion system gene was detected through polymerase chain reaction. RESULTS: Random amplified polymorphic DNA showed a genetic relatedness of approximately 50% among the tested B. pseudomallei isolates, which were grouped into two clades, of which the biggest ones comprised 18/19 isolates for primer OPQ-2, and 17/19 isolates for primer OPQ-16. Primer OPQ-4 grouped the isolates into three clades comprising 1/19, 3/19 and 15/19 isolates. Additionally, type three secretion system gene was detected in all tested isolates. CONCLUSIONS: This is an effort to type B. pseudomallei strains from Ceará, which is important for better understanding this pathogen, contributing for the epidemiological surveillance of melioidosis in this endemic region.
Authors: Dionne B Rolim; Rachel Ximenes R Lima; Ana Karoline C Ribeiro; Rafael M Colares; Leoniti D Q Lima; Alfonso J Rodríguez-Morales; Franco E Montúfar; David A B Dance Journal: Trop Med Infect Dis Date: 2018-06-05
Authors: Linda P Guamán; Carlos Barba-Ostria; Fuzhong Zhang; Edmar R Oliveira-Filho; José Gregório C Gomez; Luiziana F Silva Journal: Microb Cell Fact Date: 2018-05-15 Impact factor: 5.328