Seyedeh Elham Norollahi1, Majid Alipour2, Ali Rashidy-Pour3,4, Ali Akbar Samadani5,6, Laleh Vahedi Larijani7. 1. Faculty of Medicine, Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran. 2. Department of Biology, Islamic Azad University of Babol, Babol, Iran. 3. Faculty of Medicine, Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran. 4. Faculty of Medicine, Department of Physiology, Semnan University of Medical Sciences, Semnan, Iran. 5. Faculty of Medicine, Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran. a_a_hormoz@yahoo.com. 6. Faculty of Medicine, Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran. a_a_hormoz@yahoo.com. 7. Faculty of Medicine, Department of Pathology, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract
INTRODUCTION: Gastric cancer is one of the most serious and lethal kinds of cancer in the world. It is a multi-step, multi-factor, and elaborated process that is associated to gene abnormal expression. This study intended to investigate the WNT16 gene's expression in human gastric tumor and the margin tissues of the stomach (normal tissues). METHODS: Correspondingly, 40 samples (20 tumoral tissues and 20 non tumoral or margins tissues) were investigated in Imam Khomeini Hospital in Sari City, Mazandaran Province, Iran. In this way, real-time PCR, Taqman assay was employed to evaluate the upregulation and downregulation of this gene in both tissues in triplicate form. The GAPDH gene was selected as housekeeping gene. RESULTS: Conspicuously, the results have shown a remarkable modification in tumoral tissues, and the gene expression increased significantly in tumoral tissue. CONCLUSIONS: Conclusively, the upregulation of WNTt16 gene expression in tumoral tissues was impressive and the P value was 0.005 and the SE range was 0.064-142.154.
INTRODUCTION: Gastric cancer is one of the most serious and lethal kinds of cancer in the world. It is a multi-step, multi-factor, and elaborated process that is associated to gene abnormal expression. This study intended to investigate the WNT16 gene's expression in humangastric tumor and the margin tissues of the stomach (normal tissues). METHODS: Correspondingly, 40 samples (20 tumoral tissues and 20 non tumoral or margins tissues) were investigated in Imam Khomeini Hospital in Sari City, Mazandaran Province, Iran. In this way, real-time PCR, Taqman assay was employed to evaluate the upregulation and downregulation of this gene in both tissues in triplicate form. The GAPDH gene was selected as housekeeping gene. RESULTS: Conspicuously, the results have shown a remarkable modification in tumoral tissues, and the gene expression increased significantly in tumoral tissue. CONCLUSIONS: Conclusively, the upregulation of WNTt16 gene expression in tumoral tissues was impressive and the P value was 0.005 and the SE range was 0.064-142.154.