James Kearn1, Catherine Lilley2, Peter Urwin2, Vincent O'Connor1, Lindy Holden-Dye3. 1. Biological Sciences, Building 85, University Road, University of Southampton, Southampton SO17 1BJ, UK. 2. School of Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. 3. Biological Sciences, Building 85, University Road, University of Southampton, Southampton SO17 1BJ, UK. Electronic address: lmhd@soton.ac.uk.
Abstract
BACKGROUND: Fluensulfone is a new nematicide with an excellent profile of selective toxicity against plant parasitic nematodes. Here, its effects on the physiology and biochemistry of the potato cyst nematode Globodera pallida have been investigated and comparisons made with its effect on the life-span of the free-living nematode Caenorhabditis elegans to provide insight into its mode of action and its selective toxicity. RESULTS: Fluensulfone exerts acute effects (≤1h; ≥100μM) on stylet thrusting and motility of hatched second stage G. pallida juveniles (J2s). Chronic exposure to lower concentrations of fluensulfone (≥3days; ≤30μM), reveals a slowly developing metabolic insult in which G. pallida J2s sequentially exhibit a reduction in motility, loss of a metabolic marker for cell viability, high lipid content and tissue degeneration prior to death. These effects are absent in adults and dauers of the model genetic nematode Caenorhabditis elegans. CONCLUSION: The nematicidal action of fluensulfone follows a time-course which progresses from an early impact on motility through to an accumulating metabolic impairment, an inability to access lipid stores and death.
BACKGROUND:Fluensulfone is a new nematicide with an excellent profile of selective toxicity against plant parasitic nematodes. Here, its effects on the physiology and biochemistry of the potato cyst nematode Globodera pallida have been investigated and comparisons made with its effect on the life-span of the free-living nematode Caenorhabditis elegans to provide insight into its mode of action and its selective toxicity. RESULTS:Fluensulfone exerts acute effects (≤1h; ≥100μM) on stylet thrusting and motility of hatched second stage G. pallida juveniles (J2s). Chronic exposure to lower concentrations of fluensulfone (≥3days; ≤30μM), reveals a slowly developing metabolic insult in which G. pallida J2s sequentially exhibit a reduction in motility, loss of a metabolic marker for cell viability, high lipid content and tissue degeneration prior to death. These effects are absent in adults and dauers of the model genetic nematode Caenorhabditis elegans. CONCLUSION: The nematicidal action of fluensulfone follows a time-course which progresses from an early impact on motility through to an accumulating metabolic impairment, an inability to access lipid stores and death.
Authors: Anna Crisford; Fernando Calahorro; Elizabeth Ludlow; Jessica M C Marvin; Jennifer K Hibbard; Catherine J Lilley; James Kearn; Francesca Keefe; Peter Johnson; Rachael Harmer; Peter E Urwin; Vincent O'Connor; Lindy Holden-Dye Journal: PLoS Pathog Date: 2020-10-02 Impact factor: 6.823
Authors: Alex V Kochetov; Anastasiya A Egorova; Anastasiya Y Glagoleva; Kseniya V Strygina; Elena K Khlestkina; Sophia V Gerasimova; Natalja V Shatskaya; Gennady V Vasilyev; Dmitry A Afonnikov; Nikolay A Shmakov; Olga Y Antonova; Natalia V Alpatyeva; Alexander Khiutti; Olga S Afanasenko; Tatjana A Gavrilenko Journal: BMC Plant Biol Date: 2020-10-14 Impact factor: 4.215