| Literature DB >> 29107138 |
Jagriti Narang1, Chaitali Singhal2, Ashish Mathur3, Sachin Sharma2, Vishav Singla2, C S Pundir4.
Abstract
The present work describes the fabrication of an electrochemical paper-based analytical device (EPAD) integrated with Zn-Ag nanoblooms for detection of herpes in human, caused by Herpes virus 5(HHV-5) DNA, at the point of care. The cyclic voltammetry(CV) was used for electrochemical detection of the HHV-5 DNA in infected patient samples. The EPAD exihibited optimum current response within 5s at pH7.0 and 35°C with two dynamic linear/working ranges, 113-103 and 3×105-106copies/mL and detection limit of 97copies/mL. The device showed high selectivity, repeatability, and sensitivity. The device had many advantageous features such as portable, facile approach, economical and potential for commercialization. The proposed sensing scheme is highly feasible for future clinical detection of Herpes virus.Entities:
Keywords: Herpes virus; Methylene blue; Paper-based analytical device (EPAD); Point of care diagnosis; Zn-Ag nanoblooms
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Year: 2017 PMID： 29107138 DOI： 10.1016/j.ijbiomac.2017.10.146
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953