Determining proportions of human X and Y sperm with a recombinant deoxyribonucleic acid probe carrying a homologous sequence of sex chromosomes.

A molecular sexing assay method for human sperm DNA has been described, which is particularly suitable for estimating proportions of X and Y sperm in a large number of sample points. The method should have general applicability for testing any mammalian sperm species, since it is based on probing the homologous DNA sequences that are known to be present probably in all mammalian sex chromosomes.