Yao-Ting Huang1, Jan-Fang Cheng2, Yi-Ting Liu1, Yan-Chiao Mao3, Meng-San Wu4, Po-Yu Liu5,6. 1. Department of Computer Science & Information Engineering, National Chung Cheng University, Chia-Yi, Taiwan. 2. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA. 3. Department of Emergency Medicine, Taichung Veterans General Hospital, Taichung, Taiwan. 4. Division of Infection & Immunity, University College London, London, UK. 5. Division of Infectious Diseases, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan. 6. Rong Hsing Research Center for Translational Medicine, College of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
Abstract
AIM: Serratia marcescens wound infection after snakebite is often associated with aggressive presentations. However, the virulence determinants remain understudied. MATERIALS & METHODS: Whole-genome sequencing was performed on S. marcescens VGH107, an isolate from wound infection secondary to Trimeresurus mucrosquamatus bite. Comparative genomics approach coupled with multivirulent-locus sequencing typing was applied to systematically predict potential virulence factors. RESULTS: Multivirulent-locus sequencing typing indicated VGH107 falls within the cluster of high pathogenic strains. Comparative analysis identified virulence genes unique in VGH107, including ecpD and ecpE genes for periplasmic chaperone-pilus subunit complex and cdiA and cdiB genes for contact-dependent growth inhibition system. CONCLUSION: The data established here provide foundation for further research regarding the virulence and resistance of S. marcescens.
AIM: Serratia marcescens wound infection after snakebite is often associated with aggressive presentations. However, the virulence determinants remain understudied. MATERIALS & METHODS: Whole-genome sequencing was performed on S. marcescens VGH107, an isolate from wound infection secondary to Trimeresurus mucrosquamatus bite. Comparative genomics approach coupled with multivirulent-locus sequencing typing was applied to systematically predict potential virulence factors. RESULTS: Multivirulent-locus sequencing typing indicated VGH107 falls within the cluster of high pathogenic strains. Comparative analysis identified virulence genes unique in VGH107, including ecpD and ecpE genes for periplasmic chaperone-pilus subunit complex and cdiA and cdiB genes for contact-dependent growth inhibition system. CONCLUSION: The data established here provide foundation for further research regarding the virulence and resistance of S. marcescens.
Authors: John W A Rossen; Jill Dombrecht; Diederik Vanfleteren; Katrien De Bruyne; Alex van Belkum; Sigrid Rosema; Mariette Lokate; Erik Bathoorn; Sandra Reuter; Hajo Grundmann; Julia Ertel; Paul G Higgins; Harald Seifert Journal: J Clin Microbiol Date: 2019-03-28 Impact factor: 5.948