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Literature DB >> 291053

Isolation and characterization of germ line DNA from mouse sperm.

Abstract

Mouse germ line DNA was isolated from sperm by a physicochemical procedure that preferentially destroys contaminating somatic cell DNA. The use of reducing conditions and chelating agents in combination with phenol permitted extraction of molecular weight DNA from mature sperm nuclei with approximately 80% efficiency. Less than 0.1% somatic cell DNA contamination remained in sperm DNA prepared by this method. Germ line DNA was characterized by determination of its ultraviolet absorbance spectrum, buoyant density in cesium chloride, and melting profile on a hydroxyapatite column. Contamination by mitochondrial DNA was assessed by cesium chloride/ethidium bromide gradient centrifugation. The significance of the mouse germ line DNA isolation procedure is discussed with respect to the possible genetic transmission of mammary tumor virus and leukemia virus, the origin of antibody diversity, and the origin of testicular teratomas.

Entities: Chemical Disease Species

Mesh：

Substances：
DNA

Year: 1979 PMID： 291053 PMCID： PMC383953 DOI： 10.1073/pnas.76.8.3947

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

1. NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES.

Authors: Y MIYAZAWA; C A THOMAS
Journal: J Mol Biol Date: 1965-02 Impact factor: 5.469

2. Isolation and properties of deoxyribonucleic acid from mammalian sperm.

Authors: E BORENFREUND; E FITT; A BENDICH
Journal: Nature Date: 1961-09-30 Impact factor: 49.962

3. A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein.

Authors: K S KIRBY
Journal: Biochem J Date: 1957-07 Impact factor: 3.857

4. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

Authors: C L SCHILDKRAUT; J MARMUR; P DOTY
Journal: J Mol Biol Date: 1962-06 Impact factor: 5.469

5. Comparison of mouse mammary tumor virus-specific DNA in inbred, wild and Asian mice, and in tumors and normal organs from inbred mice.

Authors: V L Morris; E Medeiros; G M Ringold; J M Bishop; H E Varmus
Journal: J Mol Biol Date: 1977-07 Impact factor: 5.469

6. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.

Authors: J MARMUR; P DOTY
Journal: J Mol Biol Date: 1962-07 Impact factor: 5.469

7. The nucleic acids of plant tissues; the extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid.

Authors: M OGUR; G ROSEN
Journal: Arch Biochem Date: 1950-02

8. The use of molecular hybridization to track the mode of transmission and distribution of murine mammary tumor viruses: a model for etiologic studies of human breast cancer.

Authors: J Schlom; D Colcher; W Drohan; R Kettmann
Journal: Prog Exp Tumor Res Date: 1978

9. Sequences associated with intracisternal A particles are reiterated in the mouse genome.

Authors: K K Lueders; E L Kuff
Journal: Cell Date: 1977-12 Impact factor: 41.582

10. Isolation of the mouse mammary tumor virus sequences not transmitted as germinal provirus in the C3H and RIII mouse strains.

Authors: W Drohan; R Kettmann; D Colcher; J Schlom
Journal: J Virol Date: 1977-03 Impact factor: 5.103

7 in total

7. Construction of a small Mus musculus repetitive DNA library: identification of a new satellite sequence in Mus musculus.

Authors: D F Pietras; K L Bennett; L D Siracusa; M Woodworth-Gutai; V M Chapman; K W Gross; C Kane-Haas; N D Hastie
Journal: Nucleic Acids Res Date: 1983-10-25 Impact factor: 16.971

7 in total

Isolation and characterization of germ line DNA from mouse sperm.

1. NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES.

2. Isolation and properties of deoxyribonucleic acid from mammalian sperm.

3. A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein.

4. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

5. Comparison of mouse mammary tumor virus-specific DNA in inbred, wild and Asian mice, and in tumors and normal organs from inbred mice.

6. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.

7. The nucleic acids of plant tissues; the extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid.

8. The use of molecular hybridization to track the mode of transmission and distribution of murine mammary tumor viruses: a model for etiologic studies of human breast cancer.

9. Sequences associated with intracisternal A particles are reiterated in the mouse genome.

10. Isolation of the mouse mammary tumor virus sequences not transmitted as germinal provirus in the C3H and RIII mouse strains.

1. Pattern of undermethylation of the major satellite DNA of mouse sperm.

2. Variability in allelic DNA methylation in spermatozoa.

3. Variable methylation of the ribosomal RNA genes of the rat.

4. Interindividual concordance of methylation profiles in human genes for tumor necrosis factors alpha and beta.

5. Rapid method for the isolation of mammalian sperm DNA.

6. Methylation of unique sequence DNA during spermatogenesis in mice.

7. Construction of a small Mus musculus repetitive DNA library: identification of a new satellite sequence in Mus musculus.