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Literature DB >> 29101583

Therapeutic effect of 28-homobrassinolide on leukotriene synthesis in leukemia cells.

Chuan He¹, Zhigang Liu¹, Jingcao Huang¹, Ting Liu².

Abstract

The present study was aimed to investigate the effect of 28-homobrassinolide on leukotriene synthesis in the 2H3 cells. Cell viability was determined by using sulforhodamine B (SRB) assay. Leukotriene C4 (LTC4) and Leukotriene B4 (LTB4) were determined in the cell and cell lysates. Intracellular Ca2+ was determined in the intact cells. Phospholipases A2 (PLA2) expression was determined in the cells using immunofluorescence. LTC4 and LTB4 were tremendously increased in IgE-loaded 2H3 cells. However, these levels were significantly reduced following treatment with 28-homobrassinolide. The intracellular Ca2+ level was not altered by treatment. Expression of PLA2 was significantly reduced following treatment with 28-homobrassinolide. Taking all these data together, it is suggested that the 28-homobrassinolide may be a potential therapeutic agent to inhibit leukotriene synthesis in 2H3 cells.

Entities: Chemical Disease Gene

Keywords: 28-homobrassinolide; 2H3 cells; Leukotrienes; Phospholipases A2

Year: 2017 PMID： 29101583 PMCID： PMC5809672 DOI： 10.1007/s10616-017-0158-4

Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058

28 in total

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