Farzaneh Ghafarian-Alipour1, Shayan Ziaee2, Mohamad Reza Ashoori1, Mir Saeid Zakeri3, Mohammad Ali Boroumand2, Naser Aghamohammadzadeh4, Maryam Abbasi-Majdi5, Fatemeh Shool5, Navid Sarakhs Asbaghi6, Abolghasem Mohammadi7, Nosratollah Zarghami8. 1. Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 2. Department of Molecular Pathology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. 3. Department of Neurosurgery, Tabriz University of Medical Sciences, Tabriz, Iran. 4. Department of Endocrinology, Tabriz University of Medical Sciences, Tabriz, Iran. 5. Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 6. Pardis Clinical Diagnostic Laboratory, University Clinics, Tabriz University of Medical Sciences, Tabriz, Iran. 7. Department of Plant Breeding & Biotechnology, Faculty of Agriculture, University of Tabriz, Iran. 8. Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: zarghami@tbzmed.ac.ir.
Abstract
BACKGROUND AND AIMS: Recent studies show that FTO single nucleotide polymorphisms (SNPs) are associated with obesity and type 2 diabetes mellitus (T2DM). On the other hand, many animal models and clinical studies have demonstrated that apelin, an adipocytokine, is related to the obesity and T2DM. Additionally, obese women are at risk of Hyperandrogenemia. So, the aim of this study was to investigate the relationship between FTO variants (rs763967273, rs759031579, rs141115189, rs9926289, rs76804286 and rs9939609) with T2DM, serum apelin and androgenic hormones in Iranian obese women. SUBJECTS AND METHODS: 197 obese women (123 women with T2DM and 74 women as healthy control) were participated in this study. Anthropometrical and biochemical characteristics were measured. Serum apelin and androgen hormones levels were determined in 66 subjects consisting of 33 cases and 33 controls. PCR were carried out and subsequently, the PCR production was genotyped by Sanger sequencing assay. RESULTS: Our observations showed that all SNPs are related to T2DM. The rs9926289 FTO variant had a strong association with serum apelin and dehydroepiandrosterone-sulfate levels (P=0.04 and P=0.03, respectively) among SNPs. In addition, apelin and androgenic hormones were correlated with T2DM. Two polymorphisms including rs9939609 and rs9926289 had a strong Linkage disequilibrium (r2=1). CONCLUSION: FTO variants not only were associated with T2DM, but also some variants had a strong association with apelin and androgenic hormones profile.
BACKGROUND AND AIMS: Recent studies show that FTO single nucleotide polymorphisms (SNPs) are associated with obesity and type 2 diabetes mellitus (T2DM). On the other hand, many animal models and clinical studies have demonstrated that apelin, an adipocytokine, is related to the obesity and T2DM. Additionally, obesewomen are at risk of Hyperandrogenemia. So, the aim of this study was to investigate the relationship between FTO variants (rs763967273, rs759031579, rs141115189, rs9926289, rs76804286 and rs9939609) with T2DM, serum apelin and androgenic hormones in Iranian obesewomen. SUBJECTS AND METHODS: 197 obesewomen (123 women with T2DM and 74 women as healthy control) were participated in this study. Anthropometrical and biochemical characteristics were measured. Serum apelin and androgen hormones levels were determined in 66 subjects consisting of 33 cases and 33 controls. PCR were carried out and subsequently, the PCR production was genotyped by Sanger sequencing assay. RESULTS: Our observations showed that all SNPs are related to T2DM. The rs9926289FTO variant had a strong association with serum apelin and dehydroepiandrosterone-sulfate levels (P=0.04 and P=0.03, respectively) among SNPs. In addition, apelin and androgenic hormones were correlated with T2DM. Two polymorphisms including rs9939609 and rs9926289 had a strong Linkage disequilibrium (r2=1). CONCLUSION:FTO variants not only were associated with T2DM, but also some variants had a strong association with apelin and androgenic hormones profile.