Catherine Y Liu1, Masatoshi Hirayama2, Marwan Ali1, Dhara Shah1, Vinay K Aakalu1. 1. Lacrimal Cell Biology Laboratory, University of Illinois at Chicago, Department of Ophthalmology and Visual Sciences, Chicago, Illinois, USA. 2. Regulatory Biology Laboratory, Salk Institute for biological studies, San Diego, CA, USA.
Abstract
PURPOSE OF REVIEW: Aqueous deficient dry eye disease, a significant cause of morbidity worldwide, is due to dysfunction of the main and accessory lacrimal glands. Recent advances in efforts to regenerate lacrimal gland are reviewed. RECENT FINDINGS: Several strategies are being explored: ex vivo culture models of human and non-human lacrimal gland epithelial and myoepithelial cells, isolation and characterization of adult precursor cells within lacrimal glands, directed differentiation of stem cells to lacrimal gland cells, and organogenesis and engraftment techniques. SUMMARY: Conditions for primary cell culture and expansion are being established and will help in the characterization of lacrimal cells. Presumed adult precursor cells have been isolated, laying down foundations for regeneration. Stem cells have been induced to express features of lacrimal gland cells. Engraftment of ex vivo cultured lacrimal tissue is proof of concept that lacrimal gland regeneration and repopulation is possible.
PURPOSE OF REVIEW: Aqueous deficient dry eye disease, a significant cause of morbidity worldwide, is due to dysfunction of the main and accessory lacrimal glands. Recent advances in efforts to regenerate lacrimal gland are reviewed. RECENT FINDINGS: Several strategies are being explored: ex vivo culture models of human and non-human lacrimal gland epithelial and myoepithelial cells, isolation and characterization of adult precursor cells within lacrimal glands, directed differentiation of stem cells to lacrimal gland cells, and organogenesis and engraftment techniques. SUMMARY: Conditions for primary cell culture and expansion are being established and will help in the characterization of lacrimal cells. Presumed adult precursor cells have been isolated, laying down foundations for regeneration. Stem cells have been induced to express features of lacrimal gland cells. Engraftment of ex vivo cultured lacrimal tissue is proof of concept that lacrimal gland regeneration and repopulation is possible.
Authors: Philipp Ackermann; Susann Hetz; Julia Dieckow; Martin Schicht; Anja Richter; Charli Kruse; Insa S Schroeder; Matthias Jung; Friedrich P Paulsen Journal: Invest Ophthalmol Vis Sci Date: 2015-07 Impact factor: 4.799
Authors: Anna French; Christopher Bravery; James Smith; Amit Chandra; Peter Archibald; Joseph D Gold; Natalie Artzi; Hae-Won Kim; Richard W Barker; Alexander Meissner; Joseph C Wu; Jonathan C Knowles; David Williams; Guillermo García-Cardeña; Doug Sipp; Steve Oh; Jeanne F Loring; Mahendra S Rao; Brock Reeve; Ivan Wall; Andrew J Carr; Kim Bure; Glyn Stacey; Jeffrey M Karp; Evan Y Snyder; David A Brindley Journal: Stem Cells Transl Med Date: 2015-02-03 Impact factor: 6.940