Mitat Arıcıgil1, Mehmet Akif Dündar1, Abitter Yücel2, Mehmet Akif Eryılmaz1, Meryem Aktan3, Mehmet Akif Alan1, Sıdıka Fındık4, İbrahim Kılınç5. 1. a Department of Otorhinolaryngology , Meram Faculty of Medicine, Necmettin Erbakan University , Konya , Turkey. 2. b Department of Otorhinolaryngology , Horasan State Hospital , Erzurum , Turkey. 3. c Department of Radiation Oncology , Meram Faculty of Medicine, Necmettin Erbakan University , Konya , Turkey. 4. d Department of Pathology , Meram Faculty of Medicine, Necmettin Erbakan University , Konya , Turkey. 5. e Department of Medical Biochemistry , Meram Faculty of Medicine, Necmettin Erbakan University , Konya , Turkey.
Abstract
PURPOSE: We aimed to investigate the protective effect of melatonin in radiotherapy-induced thyroid gland injury in an experimental rat model. MATERIALS AND METHODS: Thirty-two rats were divided into four groups: the control group, melatonin treatment group, radiotherapy group and melatonin plus radiotherapy group. The neck region of each rat was defined by simulation and radiated with 2 Gray (Gy) per min with 6-MV photon beams, for a total dose of 18 Gy. Melatonin was administered at a dose of 50 mg/kg through intraperitoneal injection, 15 min prior to radiation exposure. Thirty days after the beginning of the study, rats were decapitated and analyses of blood and thyroid tissue were performed. RESULTS: Tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO) levels in the radiotherapy group were significantly higher than those in the melatonin plus radiotherapy group (p < .05), whereas interleukin-10 (IL-10) and glutathione (GSH) values were higher in the melatonin plus radiotherapy group (p < .05). The infiltration of inflammatory cells and percentage of apoptosis in the radiotherapy group were significantly higher than those in the melatonin plus radiotherapy group (p < .05). CONCLUSIONS: Melatonin helped protect thyroid gland structure against the undesired cytotoxic effects of radiotherapy in rats.
PURPOSE: We aimed to investigate the protective effect of melatonin in radiotherapy-induced thyroid gland injury in an experimental rat model. MATERIALS AND METHODS: Thirty-two rats were divided into four groups: the control group, melatonin treatment group, radiotherapy group and melatonin plus radiotherapy group. The neck region of each rat was defined by simulation and radiated with 2 Gray (Gy) per min with 6-MV photon beams, for a total dose of 18 Gy. Melatonin was administered at a dose of 50 mg/kg through intraperitoneal injection, 15 min prior to radiation exposure. Thirty days after the beginning of the study, rats were decapitated and analyses of blood and thyroid tissue were performed. RESULTS: Tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO) levels in the radiotherapy group were significantly higher than those in the melatonin plus radiotherapy group (p < .05), whereas interleukin-10 (IL-10) and glutathione (GSH) values were higher in the melatonin plus radiotherapy group (p < .05). The infiltration of inflammatory cells and percentage of apoptosis in the radiotherapy group were significantly higher than those in the melatonin plus radiotherapy group (p < .05). CONCLUSIONS:Melatonin helped protect thyroid gland structure against the undesired cytotoxic effects of radiotherapy in rats.
Authors: B Farhood; N H Goradel; K Mortezaee; N Khanlarkhani; E Salehi; M S Nashtaei; H Mirtavoos-Mahyari; E Motevaseli; D Shabeeb; A E Musa; M Najafi Journal: Clin Transl Oncol Date: 2018-08-22 Impact factor: 3.405