Judita Knez1,2, Katja Lakota3, Nina Božič1, Renata Okrajšek4, Nicholas Cauwenberghs2, Lutgarde Thijs2, Ivan Kneževič5, Bojan Vrtovec4, Matija Tomšič3, Saša Čučnik3, Snežna Sodin-Šemrl3, Tatiana Kuznetsova2, Jana Brguljan-Hitij1. 1. 1 Division of Internal Medicine, Department of Hypertension, University Medical Centre Ljubljana , Ljubljana, Slovenia . 2. 2 Research Unit Hypertension and Cardiovascular Epidemiology, KU Leuven Department of Cardiovascular Sciences, University of Leuven , Leuven, Belgium . 3. 3 Division of Internal Medicine, Department of Rheumatology, University Medical Centre Ljubljana , Ljubljana, Slovenia . 4. 4 Division of Internal Medicine, Department of Cardiology, University Medical Centre Ljubljana , Ljubljana, Slovenia . 5. 5 Division of Surgery, Department of Cardiovascular Surgery, University Medical Centre Ljubljana , Ljubljana, Slovenia .
Abstract
BACKGROUND/ OBJECTIVES: Heart failure (HF) is associated with disturbances in mitochondrial energy production. This mitochondrial dysfunction is reflected by depletion of mitochondrial DNA (mtDNA) in different tissues. Our aims were to determine if there was a correlation between mtDNA content measured in myocardial tissue and the easily accessible peripheral blood cells of patients with non-ischemic HF; and to determine if there was a correlation between myocardial mtDNA and left ventricular (LV) ejection fraction. METHODS: We prospectively collected paired myocardial tissue and peripheral blood samples from 13 consecutive end-stage non-ischemic HF patients undergoing cardiac transplantation. mtDNA content was assessed with real-time quantitative PCR by calculating the relative ratio of two specific mitochondrial sequences and one nuclear control gene sequence. RESULTS: HF patients with lower myocardial mtDNA content had a significantly lower LV ejection fraction (r = 0.65, p = 0.016). Peripheral blood mtDNA content correlated positively with right ventricular myocardial mtDNA content (r = 0.63, p = 0.021). We also observed that averaged myocardial DNA content tended to correlate with peripheral blood mtDNA content (r = 0.53, p = 0.061). CONCLUSIONS: In non-ischemic HF patients, myocardial mtDNA content is positively correlated with peripheral blood mtDNA content and LV function as assessed by echocardiography.
BACKGROUND/ OBJECTIVES:Heart failure (HF) is associated with disturbances in mitochondrial energy production. This mitochondrial dysfunction is reflected by depletion of mitochondrial DNA (mtDNA) in different tissues. Our aims were to determine if there was a correlation between mtDNA content measured in myocardial tissue and the easily accessible peripheral blood cells of patients with non-ischemic HF; and to determine if there was a correlation between myocardial mtDNA and left ventricular (LV) ejection fraction. METHODS: We prospectively collected paired myocardial tissue and peripheral blood samples from 13 consecutive end-stage non-ischemic HFpatients undergoing cardiac transplantation. mtDNA content was assessed with real-time quantitative PCR by calculating the relative ratio of two specific mitochondrial sequences and one nuclear control gene sequence. RESULTS: HF patients with lower myocardial mtDNA content had a significantly lower LV ejection fraction (r = 0.65, p = 0.016). Peripheral blood mtDNA content correlated positively with right ventricular myocardial mtDNA content (r = 0.63, p = 0.021). We also observed that averaged myocardial DNA content tended to correlate with peripheral blood mtDNA content (r = 0.53, p = 0.061). CONCLUSIONS: In non-ischemic HFpatients, myocardial mtDNA content is positively correlated with peripheral blood mtDNA content and LV function as assessed by echocardiography.
Authors: Di Zhao; Traci M Bartz; Nona Sotoodehnia; Wendy S Post; Susan R Heckbert; Alvaro Alonso; Ryan J Longchamps; Christina A Castellani; Yun Soo Hong; Jerome I Rotter; Henry J Lin; Brian O'Rourke; Nathan Pankratz; John A Lane; Stephanie Y Yang; Eliseo Guallar; Dan E Arking Journal: BMC Med Date: 2020-09-16 Impact factor: 11.150