Literature DB >> 29082288

Mapping RNA Sequences that Contact Viral Capsid Proteins in Virions.

C Cheng Kao1, Ella Chuang1, James Ford2, Jie Huang2, Ram Podicheti2, Doug B Rusch2.   

Abstract

We have adapted the methodology of CLIP-seq (Crosslinking-Immunoprecipitation and DNA Sequencing) to map the segments of encapsidated RNAs that contact the protein shells of virions. Results from the protocol report on the RNA sequences that contact the viral capsid.

Entities:  

Keywords:  CLIP-Seq; Protein-RNA interaction; RNA virus; Viral capsid; Virion assembly

Year:  2014        PMID: 29082288      PMCID: PMC5659631          DOI: 10.21769/BioProtoc.2398

Source DB:  PubMed          Journal:  Bio Protoc        ISSN: 2331-8325


  7 in total

1.  JBrowse: a next-generation genome browser.

Authors:  Mitchell E Skinner; Andrew V Uzilov; Lincoln D Stein; Christopher J Mungall; Ian H Holmes
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2.  Fast gapped-read alignment with Bowtie 2.

Authors:  Ben Langmead; Steven L Salzberg
Journal:  Nat Methods       Date:  2012-03-04       Impact factor: 28.547

3.  The tripartite virions of the brome mosaic virus have distinct physical properties that affect the timing of the infection process.

Authors:  Robert Vaughan; Brady Tragesser; Peng Ni; Xiang Ma; Bogdan Dragnea; C Cheng Kao
Journal:  J Virol       Date:  2014-03-26       Impact factor: 5.103

4.  Phosphorylation of the Brome Mosaic Virus Capsid Regulates the Timing of Viral Infection.

Authors:  Haley S Hoover; Joseph Che-Yen Wang; Stefani Middleton; Peng Ni; Adam Zlotnick; Robert C Vaughan; C Cheng Kao
Journal:  J Virol       Date:  2016-08-12       Impact factor: 5.103

5.  The plant host can affect the encapsidation of brome mosaic virus (BMV) RNA: BMV virions are surprisingly heterogeneous.

Authors:  Peng Ni; Robert C Vaughan; Brady Tragesser; Haley Hoover; C Cheng Kao
Journal:  J Mol Biol       Date:  2013-09-13       Impact factor: 5.469

6.  Trimmomatic: a flexible trimmer for Illumina sequence data.

Authors:  Anthony M Bolger; Marc Lohse; Bjoern Usadel
Journal:  Bioinformatics       Date:  2014-04-01       Impact factor: 6.937

7.  Direct Evidence for Packaging Signal-Mediated Assembly of Bacteriophage MS2.

Authors:  Óttar Rolfsson; Stefani Middleton; Iain W Manfield; Simon J White; Baochang Fan; Robert Vaughan; Neil A Ranson; Eric Dykeman; Reidun Twarock; James Ford; C Cheng Kao; Peter G Stockley
Journal:  J Mol Biol       Date:  2015-12-01       Impact factor: 5.469

  7 in total

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