Literature DB >> 29082276

Analysis of Protein Stability by the Cycloheximide Chase Assay.

Shih-Han Kao1, Wen-Lung Wang2, Chi-Yuan Chen3, Yih-Leong Chang4, Yi-Ying Wu5, Yi-Ting Wang6,7,8, Shu-Ping Wang9, Alexey I Nesvizhskii10,11, Yu-Ju Chen6,7, Tse-Ming Hong5, Pan-Chyr Yang2,12,13.   

Abstract

Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly used in cell biology in terms of determining the half-life of a given protein and has gained much popularity in cancer research. Here we present a full cycloheximide chase assay in our laboratory using a lung adenocarcinoma cell line, CL1-5, as a model.

Entities:  

Year:  2015        PMID： 29082276      PMCID： PMC5659619          DOI： 10.21769/BioProtoc.1374

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  2 in total

1.  GSK3β controls epithelial-mesenchymal transition and tumor metastasis by CHIP-mediated degradation of Slug.

Authors:  S-H Kao; W-L Wang; C-Y Chen; Y-L Chang; Y-Y Wu; Y-T Wang; S-P Wang; A I Nesvizhskii; Y-J Chen; T-M Hong; P-C Yang
Journal:  Oncogene       Date:  2013-07-15       Impact factor: 9.867

2.  p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug.

Authors:  Shu-Ping Wang; Wen-Lung Wang; Yih-Leong Chang; Chen-Tu Wu; Yu-Chih Chao; Shih-Han Kao; Ang Yuan; Chung-Wu Lin; Shuenn-Chen Yang; Wing-Kai Chan; Ker-Chau Li; Tse-Ming Hong; Pan-Chyr Yang
Journal:  Nat Cell Biol       Date:  2009-05-17       Impact factor: 28.824
  2 in total
  24 in total

1.  SUMO Modification Stabilizes Enterovirus 71 Polymerase 3D To Facilitate Viral Replication.

Authors:  Yan Liu; Zhenhua Zheng; Bo Shu; Jin Meng; Yuan Zhang; Caishang Zheng; Xianliang Ke; Peng Gong; Qinxue Hu; Hanzhong Wang
Journal:  J Virol       Date:  2016-11-14       Impact factor: 5.103

2.  A Nonpump Function of Sodium Iodide Symporter in Thyroid Cancer via Cross-talk with PTEN Signaling.

Authors:  Fang Feng; Lamis Yehia; Ying Ni; Yi Seok Chang; Sissy Meihua Jhiang; Charis Eng
Journal:  Cancer Res       Date:  2018-09-14       Impact factor: 12.701

3.  A robust and economical pulse-chase protocol to measure the turnover of HaloTag fusion proteins.

Authors:  Ronald A Merrill; Jianing Song; Rikki A Kephart; Annette J Klomp; Claire E Noack; Stefan Strack
Journal:  J Biol Chem       Date:  2019-09-11       Impact factor: 5.157

4.  Evaluating Cell Membrane Localization and Intracellular Transport of Proteins by Biotinylation.

Authors:  Eduardo Reyes-Alvarez; Timothy J Walker; Lois M Mulligan
Journal:  Methods Mol Biol       Date:  2022

5.  Assessment of fluorescent protein candidates for multi-color flow cytometry analysis of Saccharomyces cerevisiae.

Authors:  Raquel Perruca-Foncillas; Johan Davidsson; Magnus Carlquist; Marie F Gorwa-Grauslund
Journal:  Biotechnol Rep (Amst)       Date:  2022-04-26

6.  Loss of Function Mutation in ELF4 Causes Autoinflammatory and Immunodeficiency Disease in Human.

Authors:  Gan Sun; Luyao Qiu; Lang Yu; Yunfei An; Yuan Ding; Lina Zhou; Junfeng Wu; Xi Yang; Zhiyong Zhang; Xuemei Tang; Huawei Xia; Lili Cao; Fuping You; Xiaodong Zhao; Hongqiang Du
Journal:  J Clin Immunol       Date:  2022-03-09       Impact factor: 8.542

7.  MicroRNA-26a/Death-Associated Protein Kinase 1 Signaling Induces Synucleinopathy and Dopaminergic Neuron Degeneration in Parkinson's Disease.

Authors:  Ying Su; Man-Fei Deng; Wan Xiong; Ao-Ji Xie; Jifeng Guo; Zhi-Hou Liang; Bo Hu; Jian-Guo Chen; Xiongwei Zhu; Heng-Ye Man; Youming Lu; Dan Liu; Beisha Tang; Ling-Qiang Zhu
Journal:  Biol Psychiatry       Date:  2018-12-19       Impact factor: 13.382

8.  Increased AT1 receptor expression mediates vasoconstriction leading to hypertension in Snx1-/- mice.

Authors:  Chao Liu; Xingyue Li; Jinjuan Fu; Ken Chen; Qiao Liao; Jialiang Wang; Caiyu Chen; Hao Luo; Pedro A Jose; Yongjian Yang; Jian Yang; Chunyu Zeng
Journal:  Hypertens Res       Date:  2021-05-10       Impact factor: 3.872

9.  Mixed Lineage Kinase 3 phosphorylates prolyl-isomerase PIN1 and potentiates GLI1 signaling in pancreatic cancer development.

Authors:  Navin Viswakarma; Gautam Sondarva; Daniel R Principe; Rakesh Sathish Nair; Sandeep Kumar; Sunil Kumar Singh; Subhasis Das; Subhash C Sinha; Paul J Grippo; Sam Grimaldo; Pier Cristoforo Giulianotti; Basabi Rana; Ajay Rana
Journal:  Cancer Lett       Date:  2021-05-27       Impact factor: 9.756

10.  A novel positive feedback-loop between the HTLV-1 oncoprotein Tax and NF-κB activity in T-cells.

Authors:  Sebastian Millen; Lina Meretuk; Tim Göttlicher; Sarah Schmitt; Bernhard Fleckenstein; Andrea K Thoma-Kress
Journal:  Retrovirology       Date:  2020-09-10       Impact factor: 4.602
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.