Henok A Tegegne1, Ivana Koláčková2, Renata Karpíšková2. 1. Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic; Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. Electronic address: henokayalew79@gmail.com. 2. Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic.
Abstract
OBJECTIVE: To evaluate the diversity and molecular characteristics of livestock associated methicillin-resistant Staphylococcus aureus in livestock animals, food of animal origin and the environment in the Czech Republic. METHODS: After having been primarily enriched in buffered peptone water, the samples were cultured on Baird-Parker agar. Presumptive colonies were sub-cultured to blood agar and assessed morphologically. Furthermore, presumptive Staphylococcus aureus colonies were confirmed by MALDI-TOF. Multiplex PCR, spa-typing, and MLST have been used to characterize the strains. Each mecA-positive Staphylococcus aureus isolates were examined against 14 different antimicrobials by using disk diffusion method. RESULTS: In this study, 13 different spa-types belonging to five sequence types (ST) were detected. Ninety four percent of tested strains belonged to CC/ST398 for which t011, t034, t2123 and t2346 were the vast major spa-types. In addition, non-ST398 clones such as CC1(t127), ST5(t3598), ST8(t064) and ST361(t315) were detected, which are known as human associated clones. CONCLUSIONS: The diversity of livestock associated methicillin-resistant Staphylococcus aureus has grown, and detecting lineages of human origin in animals and vice-versa becomes more common. Thus, livestock animal and its products will be a potential for the evolvement of methicillin-resistant Staphylococcus aureus in human population. Monitoring of pigs as well as other food-producing animal species and their products is therefore recommended.
OBJECTIVE: To evaluate the diversity and molecular characteristics of livestock associated methicillin-resistant Staphylococcus aureus in livestock animals, food of animal origin and the environment in the Czech Republic. METHODS: After having been primarily enriched in buffered peptone water, the samples were cultured on Baird-Parker agar. Presumptive colonies were sub-cultured to blood agar and assessed morphologically. Furthermore, presumptive Staphylococcus aureus colonies were confirmed by MALDI-TOF. Multiplex PCR, spa-typing, and MLST have been used to characterize the strains. Each mecA-positive Staphylococcus aureus isolates were examined against 14 different antimicrobials by using disk diffusion method. RESULTS: In this study, 13 different spa-types belonging to five sequence types (ST) were detected. Ninety four percent of tested strains belonged to CC/ST398 for which t011, t034, t2123 and t2346 were the vast major spa-types. In addition, non-ST398 clones such as CC1(t127), ST5(t3598), ST8(t064) and ST361(t315) were detected, which are known as human associated clones. CONCLUSIONS: The diversity of livestock associated methicillin-resistant Staphylococcus aureus has grown, and detecting lineages of human origin in animals and vice-versa becomes more common. Thus, livestock animal and its products will be a potential for the evolvement of methicillin-resistant Staphylococcus aureus in human population. Monitoring of pigs as well as other food-producing animal species and their products is therefore recommended.
Authors: Mulemba Tillika Samutela; Bruno Stephen July Phiri; Edgar Simulundu; Geoffrey Kwenda; Ladslav Moonga; Eugene C Bwalya; Walter Muleya; Therese Nyirahabimana; Kaunda Yamba; Henson Kainga; Simegnew Adugna Kallu; Innocent Mwape; Andrew Frey; Matthew Bates; Hideaki Higashi; Bernard Mudenda Hang'ombe Journal: Antibiotics (Basel) Date: 2022-06-24