Literature DB >> 29080129

Quantitative Live Cell FLIM Imaging in Three Dimensions.

Alix Le Marois1, Klaus Suhling2.   

Abstract

In this chapter, the concept of fluorescence lifetime and its utility in quantitative live cell imaging will be introduced, along with methods to record and analyze FLIM data. Relevant applications in 3D tissue and live cell imaging, including multiplexed FLIM detection, will also be detailed.

Keywords:  FLIM data analysis; FLIM techniques; Multi-channel FLIM; Multi-modal FLIM; Optical sectioning; Time-correlated single photon counting (TCSPC)

Mesh：

Substances：

Year:  2017        PMID： 29080129     DOI： 10.1007/978-3-319-67358-5_3

Source DB:  PubMed          Journal:  Adv Exp Med Biol        ISSN： 0065-2598            Impact factor:   2.622


  7 in total

1.  NAD(P)H autofluorescence lifetime imaging enables single cell analyses of cellular metabolism of osteoblasts in vitro and in vivo via two-photon microscopy.

Authors:  Kevin Schilling; Edward Brown; Xinping Zhang
Journal:  Bone       Date:  2021-11-13       Impact factor: 4.398

Review 2.  Imaging of oxygen and hypoxia in cell and tissue samples.

Authors:  Dmitri B Papkovsky; Ruslan I Dmitriev
Journal:  Cell Mol Life Sci       Date:  2018-05-14       Impact factor: 9.261

3.  Luminescence lifetime imaging of three-dimensional biological objects.

Authors:  Ruslan I Dmitriev; Xavier Intes; Margarida M Barroso
Journal:  J Cell Sci       Date:  2021-05-07       Impact factor: 5.285

4.  Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies.

Authors:  Magalie Bénard; Damien Schapman; Christophe Chamot; Fatéméh Dubois; Guénaëlle Levallet; Hitoshi Komuro; Ludovic Galas
Journal:  Int J Mol Sci       Date:  2021-10-14       Impact factor: 5.923

5.  Toxicity of fungicides to Pisum sativum: a study of oxidative damage, growth suppression, cellular death and morpho-anatomical changes.

Authors:  Mohammad Shahid; Bilal Ahmed; Almas Zaidi; Mohd Saghir Khan
Journal:  RSC Adv       Date:  2018-11-15       Impact factor: 4.036

6.  Visualization of integrin molecules by fluorescence imaging and techniques.

Authors:  Chen Cai; Hao Sun; Liang Hu; Zhichao Fan
Journal:  Biocell       Date:  2021-02-19       Impact factor: 1.254

7.  Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell immobilization.

Authors:  K Sowri Babu; Pedro F Pinheiro; Cátia F Marques; Gonçalo C Justino; Suzana M Andrade; Marta M Alves
Journal:  Sci Rep       Date:  2020-09-14       Impact factor: 4.379
  7 in total

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