Y-B Yang1, X-L Wu, B Ke, Y-J Huang, S-Q Chen, Y-Q Su, J Qin. 1. Department of Traditional Chinese Medicine, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong Province, Chin. myxs32@126.com.cn.
Abstract
OBJECTIVE: To investigate the effect of caloric restriction (CR) on expressions of peroxisome proliferators-activated receptors (PPARs) and positive transcription elongation factor b (P-TEFb) (including cyclin-dependent kinase 9 (CDK9) and cyclin T1) protein in visceral adipose tissue of obese rats. MATERIALS AND METHODS: Obese rats were induced by high-fat diet for 8 weeks. Then they were divided into three groups: Model (n=5), 50% Calorie Restricted (50% CR, n=5), Intermittent Fasting (IF) (eight cycles of 3-d fasting and 3-d refeeding, n=6) for 8 weeks. Biochemical parameters were measured. Protein and mRNA expression of Cdk9, cyclin T1 and PPARs were qualified in visceral adipose tissue. RESULTS: A significant decline in fasting plasma glucose (FPG), homeostatic model assessment of insulin resistance (HOMA-IR), body weight, and visceral fat weight was observed in 50% CR group. The IF group exhibited a significant decrease in FPG, HOMA-IR, visceral fat weight. Both 50% CR and IF down-regulated mRNA and protein expression of PPARγ and Cdk9, cyclin T1 and up-regulated mRNA and protein expression of PPARβ. CONCLUSIONS: These results suggest that the effects of 50% CR and IF on HOMA-IR, body weight, visceral fat weight, P-TEFb and PPARγ expression may be related to their protective potential on obesity.
OBJECTIVE: To investigate the effect of caloric restriction (CR) on expressions of peroxisome proliferators-activated receptors (PPARs) and positive transcription elongation factor b (P-TEFb) (including cyclin-dependent kinase 9 (CDK9) and cyclin T1) protein in visceral adipose tissue of obeserats. MATERIALS AND METHODS:Obeserats were induced by high-fat diet for 8 weeks. Then they were divided into three groups: Model (n=5), 50% Calorie Restricted (50% CR, n=5), Intermittent Fasting (IF) (eight cycles of 3-d fasting and 3-d refeeding, n=6) for 8 weeks. Biochemical parameters were measured. Protein and mRNA expression of Cdk9, cyclin T1 and PPARs were qualified in visceral adipose tissue. RESULTS: A significant decline in fasting plasma glucose (FPG), homeostatic model assessment of insulin resistance (HOMA-IR), body weight, and visceral fat weight was observed in 50% CR group. The IF group exhibited a significant decrease in FPG, HOMA-IR, visceral fat weight. Both 50% CR and IF down-regulated mRNA and protein expression of PPARγ and Cdk9, cyclin T1 and up-regulated mRNA and protein expression of PPARβ. CONCLUSIONS: These results suggest that the effects of 50% CR and IF on HOMA-IR, body weight, visceral fat weight, P-TEFb and PPARγ expression may be related to their protective potential on obesity.