Literature DB >> 29076097

Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines.

Divya S Vinjamur1, Daniel E Bauer2,3.   

Abstract

The recently established human umbilical cord blood-derived erythroid progenitor (HUDEP) cell lines have equipped red blood cell researchers with valuable in vitro models of erythroid development. Of the three established HUDEP cell lines, HUDEP-2 cells express predominantly adult β-globin and most closely resemble adult erythroid cells. This chapter describes culture protocols for the maintenance and erythroid differentiation of HUDEP-2 cells. Methods to genetically manipulate HUDEP-2 cells using a CRISPR/Cas9 nuclease-based approach are also discussed.

Entities:  

Keywords:  CRISPR/Cas9; Erythroid differentiation; Fetal hemoglobin; HUDEP; Hemoglobin switching; Immortalized erythroid cell line

Mesh：

Substances：

Year:  2018        PMID： 29076097     DOI： 10.1007/978-1-4939-7428-3_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Comparing the two leading erythroid lines BEL-A and HUDEP-2.

Authors:  Deborah E Daniels; Damien J Downes; Ivan Ferrer-Vicens; Daniel C J Ferguson; Belinda K Singleton; Marieangela C Wilson; Kongtana Trakarnsanga; Ryo Kurita; Yukio Nakamura; David J Anstee; Jan Frayne
Journal:  Haematologica       Date:  2019-11-21       Impact factor: 9.941

2.  Correction of β-thalassemia by CRISPR/Cas9 editing of the α-globin locus in human hematopoietic stem cells.

Authors:  Giulia Pavani; Anna Fabiano; Marine Laurent; Fatima Amor; Erika Cantelli; Anne Chalumeau; Giulia Maule; Alexandra Tachtsidi; Jean-Paul Concordet; Anna Cereseto; Fulvio Mavilio; Giuliana Ferrari; Annarita Miccio; Mario Amendola
Journal:  Blood Adv       Date:  2021-03-09

3.  Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells.

Authors:  Stuti Mehta; Altantsetseg Buyanbat; Yan Kai; Ozge Karayel; Seth Raphael Goldman; Davide Seruggia; Kevin Zhang; Yuko Fujiwara; Katherine A Donovan; Qian Zhu; Huan Yang; Behnam Nabet; Nathanael S Gray; Matthias Mann; Eric S Fischer; Karen Adelman; Stuart H Orkin
Journal:  Cell Chem Biol       Date:  2022-07-14       Impact factor: 9.039

4.  Hemogen/BRG1 cooperativity modulates promoter and enhancer activation during erythropoiesis.

Authors:  Xiang Guo; Yangu Zhao; Juhyun Kim; Ann Dean
Journal:  Blood       Date:  2022-06-16       Impact factor: 25.476

5.  Common variants in signaling transcription-factor-binding sites drive phenotypic variability in red blood cell traits.

Authors:  Avik Choudhuri; Eirini Trompouki; Brian J Abraham; Leandro M Colli; Kian Hong Kock; William Mallard; Min-Lee Yang; Divya S Vinjamur; Alireza Ghamari; Audrey Sporrij; Karen Hoi; Barbara Hummel; Sonja Boatman; Victoria Chan; Sierra Tseng; Satish K Nandakumar; Song Yang; Asher Lichtig; Michael Superdock; Seraj N Grimes; Teresa V Bowman; Yi Zhou; Shinichiro Takahashi; Roby Joehanes; Alan B Cantor; Daniel E Bauer; Santhi K Ganesh; John Rinn; Paul S Albert; Martha L Bulyk; Stephen J Chanock; Richard A Young; Leonard I Zon
Journal:  Nat Genet       Date:  2020-11-23       Impact factor: 38.330

6.  Whole-genome sequencing association analysis of quantitative red blood cell phenotypes: The NHLBI TOPMed program.

Authors:  Yao Hu; Adrienne M Stilp; Caitlin P McHugh; Shuquan Rao; Deepti Jain; Xiuwen Zheng; John Lane; Sébastian Méric de Bellefon; Laura M Raffield; Ming-Huei Chen; Lisa R Yanek; Marsha Wheeler; Yao Yao; Chunyan Ren; Jai Broome; Jee-Young Moon; Paul S de Vries; Brian D Hobbs; Quan Sun; Praveen Surendran; Jennifer A Brody; Thomas W Blackwell; Hélène Choquet; Kathleen Ryan; Ravindranath Duggirala; Nancy Heard-Costa; Zhe Wang; Nathalie Chami; Michael H Preuss; Nancy Min; Lynette Ekunwe; Leslie A Lange; Mary Cushman; Nauder Faraday; Joanne E Curran; Laura Almasy; Kousik Kundu; Albert V Smith; Stacey Gabriel; Jerome I Rotter; Myriam Fornage; Donald M Lloyd-Jones; Ramachandran S Vasan; Nicholas L Smith; Kari E North; Eric Boerwinkle; Lewis C Becker; Joshua P Lewis; Goncalo R Abecasis; Lifang Hou; Jeffrey R O'Connell; Alanna C Morrison; Terri H Beaty; Robert Kaplan; Adolfo Correa; John Blangero; Eric Jorgenson; Bruce M Psaty; Charles Kooperberg; Russell T Walton; Benjamin P Kleinstiver; Hua Tang; Ruth J F Loos; Nicole Soranzo; Adam S Butterworth; Debbie Nickerson; Stephen S Rich; Braxton D Mitchell; Andrew D Johnson; Paul L Auer; Yun Li; Rasika A Mathias; Guillaume Lettre; Nathan Pankratz; Cathy C Laurie; Cecelia A Laurie; Daniel E Bauer; Matthew P Conomos; Alexander P Reiner
Journal:  Am J Hum Genet       Date:  2021-04-21       Impact factor: 11.025

7.  ZNF410 represses fetal globin by singular control of CHD4.

Authors:  Divya S Vinjamur; Qiuming Yao; Mitchel A Cole; Connor McGuckin; Chunyan Ren; Jing Zeng; Mir Hossain; Kevin Luk; Scot A Wolfe; Luca Pinello; Daniel E Bauer
Journal:  Nat Genet       Date:  2021-04-15       Impact factor: 38.330

8.  Robust CRISPR/Cas9 Genome Editing of the HUDEP-2 Erythroid Precursor Line Using Plasmids and Single-Stranded Oligonucleotide Donors.

Authors:  Gemma Moir-Meyer; Pak Leng Cheong; Aude-Anais Olijnik; Jill Brown; Samantha Knight; Andrew King; Ryo Kurita; Yukio Nakamura; Richard J Gibbons; Douglas R Higgs; Veronica J Buckle; Christian Babbs
Journal:  Methods Protoc       Date:  2018-07-30

9.  Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytes.

Authors:  Yu Jin Park; Su-Hee Jeon; Hyun-Kyung Kim; Eun Jung Suh; Seung Jun Choi; Sinyoung Kim; Hyun Ok Kim
Journal:  J Transl Med       Date:  2020-06-12       Impact factor: 5.531

10.  Direct Generation of Immortalized Erythroid Progenitor Cell Lines from Peripheral Blood Mononuclear Cells.

Authors:  Abhirup Bagchi; Aneesha Nath; Vasanth Thamodaran; Smitha Ijee; Dhavapriya Palani; Vignesh Rajendiran; Vigneshwaran Venkatesan; Phaneendra Datari; Aswin Anand Pai; Nancy Beryl Janet; Poonkuzhali Balasubramanian; Yukio Nakamura; Alok Srivastava; Kumarasamypet Murugesan Mohankumar; Saravanabhavan Thangavel; Shaji R Velayudhan
Journal:  Cells       Date:  2021-03-01       Impact factor: 6.600
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