Cody T Haun1, Michael D Roberts1,2, Matthew A Romero1, Shelby C Osburn1, James C Healy2, Angelique N Moore2,3, Christopher B Mobley1, Paul A Roberson1, Wesley C Kephart1, Petey W Mumford1, Michael D Goodlett2,4, David D Pascoe1, Jeffrey S Martin5,6. 1. School of Kinesiology, Auburn University, Auburn, AL, USA. 2. Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine, Auburn Campus, 910 S. Donahue Drive, Auburn, AL, 36832, USA. 3. College of Human Sciences, Auburn University, Auburn, AL, 36849, USA. 4. Athletics Department, Auburn University, Auburn, AL, USA. 5. School of Kinesiology, Auburn University, Auburn, AL, USA. jmartin@auburn.vcom.edu. 6. Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine, Auburn Campus, 910 S. Donahue Drive, Auburn, AL, 36832, USA. jmartin@auburn.vcom.edu.
Abstract
PURPOSE: To compare the effects of external pneumatic compression (EPC) and sham when used concurrently with high intensity interval training (HIIT) on performance-related outcomes and recovery-related molecular measures. METHODS:Eighteen recreationally endurance-trained male participants (age: 21.6 ± 2.4 years, BMI: 25.7 ± 0.5 kg/m2, VO2peak: 51.3 ± 0.9 mL/kg/min) were randomized to balanced sham and EPC treatment groups. Three consecutive days of HIIT followed by EPC/sham treatment (Days 2-4) and 3 consecutive days of recovery (Days 5-7) with EPC/sham only on Days 5-6 were employed. Venipuncture, flexibility and pressure-to-pain threshold (PPT) measurements were made throughout. Vastus lateralis muscle was biopsied at PRE (i.e., Day 1), 1-h post-EPC/sham treatment on Day 2 (POST1), and 24-h post-EPC/sham treatment on Day 7 (POST2). 6-km run time trial performance was tested at PRE and POST2. RESULTS: No group × time interaction was observed for flexibility, PPT, or serum measures of creatine kinase (CK), hsCRP, and8-isoprostane. However, there was a main effect of time for serum CK (p = 0.005). Change from PRE in 6-km run times at POST2 were not significantly different between groups. Significant between-groups differences existed for change from PRE in atrogin-1 mRNA (p = 0.018) at the POST1 time point (EPC: - 19.7 ± 8.1%, sham: + 7.7 ± 5.9%) and atrogin-1 protein concentration (p = 0.013) at the POST2 time point (EPC: - 31.8 ± 7.5%, sham: + 96.0 ± 34.7%). In addition, change from PRE in poly-Ub proteins was significantly different between groups at both the POST1 (EPC: - 26.0 ± 10.3%, sham: + 34.8 ± 28.5%; p = 0.046) and POST2 (EPC: - 33.7 ± 17.2%, sham: + 21.4 ± 14.9%; p = 0.037) time points. CONCLUSIONS:EPC when used concurrently with HIIT and in subsequent recovery days reduces skeletal muscle markers of proteolysis.
RCT Entities:
PURPOSE: To compare the effects of external pneumatic compression (EPC) and sham when used concurrently with high intensity interval training (HIIT) on performance-related outcomes and recovery-related molecular measures. METHODS: Eighteen recreationally endurance-trained male participants (age: 21.6 ± 2.4 years, BMI: 25.7 ± 0.5 kg/m2, VO2peak: 51.3 ± 0.9 mL/kg/min) were randomized to balanced sham and EPC treatment groups. Three consecutive days of HIIT followed by EPC/sham treatment (Days 2-4) and 3 consecutive days of recovery (Days 5-7) with EPC/sham only on Days 5-6 were employed. Venipuncture, flexibility and pressure-to-pain threshold (PPT) measurements were made throughout. Vastus lateralis muscle was biopsied at PRE (i.e., Day 1), 1-h post-EPC/sham treatment on Day 2 (POST1), and 24-h post-EPC/sham treatment on Day 7 (POST2). 6-km run time trial performance was tested at PRE and POST2. RESULTS: No group × time interaction was observed for flexibility, PPT, or serum measures of creatine kinase (CK), hsCRP, and 8-isoprostane. However, there was a main effect of time for serum CK (p = 0.005). Change from PRE in 6-km run times at POST2 were not significantly different between groups. Significant between-groups differences existed for change from PRE in atrogin-1 mRNA (p = 0.018) at the POST1 time point (EPC: - 19.7 ± 8.1%, sham: + 7.7 ± 5.9%) and atrogin-1 protein concentration (p = 0.013) at the POST2 time point (EPC: - 31.8 ± 7.5%, sham: + 96.0 ± 34.7%). In addition, change from PRE in poly-Ub proteins was significantly different between groups at both the POST1 (EPC: - 26.0 ± 10.3%, sham: + 34.8 ± 28.5%; p = 0.046) and POST2 (EPC: - 33.7 ± 17.2%, sham: + 21.4 ± 14.9%; p = 0.037) time points. CONCLUSIONS: EPC when used concurrently with HIIT and in subsequent recovery days reduces skeletal muscle markers of proteolysis.
Authors: Jeffrey S Martin; Zachary D Friedenreich; Alexandra R Borges; Michael D Roberts Journal: Appl Physiol Nutr Metab Date: 2015-08-19 Impact factor: 2.665
Authors: J S Martin; W C Kephart; C B Mobley; T J Wilson; M D Goodlett; M D Roberts Journal: Clin Physiol Funct Imaging Date: 2016-01-14 Impact factor: 2.273
Authors: Wesley C Kephart; C Brooks Mobley; Carlton D Fox; David D Pascoe; JoEllen M Sefton; Trent J Wilson; Michael D Goodlett; Andreas N Kavazis; Michael D Roberts; Jeffrey S Martin Journal: Exp Physiol Date: 2015-06-17 Impact factor: 2.969