Kelsey H Collins1,2,3, Walter Herzog1,2,3, Raylene A Reimer1,4, Carol R Reno2, Bryan J Heard2, David A Hart5,6,7,8,9,10. 1. Human Performance Laboratory, Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada. 2. McCaig Institute for Bone and Joint Health, University of Calgary, Calgary, AB, Canada. 3. Biomedical Engineering Program, University of Calgary, Calgary, AB, Canada. 4. Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB, Canada. 5. Human Performance Laboratory, Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada. hartd@ucalgary.ca. 6. McCaig Institute for Bone and Joint Health, University of Calgary, Calgary, AB, Canada. hartd@ucalgary.ca. 7. The Centre for Hip Health and Mobility, Department of Family Practice, University of British Columbia, Vancouver, BC, Canada. hartd@ucalgary.ca. 8. Biomedical Engineering Program, University of Calgary, Calgary, AB, Canada. hartd@ucalgary.ca. 9. Alberta Health Services Bone and Joint Health Strategic Clinical Network, Calgary, Canada. hartd@ucalgary.ca. 10. , 3A22 HRIC 3330 Hospital Drive NW, Calgary, T2N 4N1, AB, Canada. hartd@ucalgary.ca.
Abstract
OBJECTIVE AND DESIGN: The purpose of this study was to investigate if diet-induced obesity (DIO) and subsequent low-level systemic inflammation would result in local increases in pro-inflammatory mediators in the vitreous humour (VH) of the eyes of rats. METHODS: Sixteen male Sprague-Dawley rats were fed a high-fat/high-sucrose (n = 9) or chow control-diet (n = 7) for 12-weeks. RT-qPCR was conducted on RNA from VH cells and a 27-plex Luminex® Assay was conducted on VH fluid and serum. RESULTS: Increased protein levels for IL-1β, IL-6, and IL-18 in both serum and VH fluid were observed. VH protein levels for IL-13 and IL-17 were also increased. All mediators significantly increased in VH fluid were also positively correlated with percent body fat. Increased mRNA levels in VH cells for an oxidative stress molecule were accompanied by decreased mRNA levels for an antioxidant scavenger, suggesting an antioxidant/oxidant imbalance in the VH with DIO. In addition, decreased mRNA levels for TRAIL, FAS-L and TGF-β, molecules associated with immune privilege, were also significantly depressed. CONCLUSIONS: DIO-related metabolic disturbances disrupt VH homeostasis in a manner that reflects development of a pro-inflammatory environment. Prolonged exposure to such an environment may lead to overt pathologies with compromised eye function.
OBJECTIVE AND DESIGN: The purpose of this study was to investigate if diet-induced obesity (DIO) and subsequent low-level systemic inflammation would result in local increases in pro-inflammatory mediators in the vitreous humour (VH) of the eyes of rats. METHODS: Sixteen male Sprague-Dawley rats were fed a high-fat/high-sucrose (n = 9) or chow control-diet (n = 7) for 12-weeks. RT-qPCR was conducted on RNA from VH cells and a 27-plex Luminex® Assay was conducted on VH fluid and serum. RESULTS: Increased protein levels for IL-1β, IL-6, and IL-18 in both serum and VH fluid were observed. VH protein levels for IL-13 and IL-17 were also increased. All mediators significantly increased in VH fluid were also positively correlated with percent body fat. Increased mRNA levels in VH cells for an oxidative stress molecule were accompanied by decreased mRNA levels for an antioxidant scavenger, suggesting an antioxidant/oxidant imbalance in the VH with DIO. In addition, decreased mRNA levels for TRAIL, FAS-L and TGF-β, molecules associated with immune privilege, were also significantly depressed. CONCLUSIONS:DIO-related metabolic disturbances disrupt VH homeostasis in a manner that reflects development of a pro-inflammatory environment. Prolonged exposure to such an environment may lead to overt pathologies with compromised eye function.
Authors: Andrea M Vincent; James W Russell; Kelli A Sullivan; Carey Backus; John M Hayes; Lisa L McLean; Eva L Feldman Journal: Exp Neurol Date: 2007-08-03 Impact factor: 5.330
Authors: Julianne C Barry; Soroush Shakibakho; Cody Durrer; Svetlana Simtchouk; Kamaldeep K Jawanda; Sylvia T Cheung; Alice L Mui; Jonathan P Little Journal: Sci Rep Date: 2016-02-17 Impact factor: 4.379
Authors: Kelsey H Collins; Graham Z MacDonald; David A Hart; Ruth A Seerattan; Jaqueline L Rios; Raylene A Reimer; Walter Herzog Journal: J Sport Health Sci Date: 2019-06-15 Impact factor: 7.179