Literature DB >> 29075655

Purification of FLAG-tagged Secreted Proteins from Mammalian Cells.

Eisuke Itakura1, Changchun Chen2, Mario de Bono2.   

Abstract

This protocol describes a method for purifying glycosylated FLAG-tagged secreted proteins with disulfide bonds from mammalian cells. The purified products can be used for various applications, such as ligand binding assays.

Entities:  

Keywords:  Ligand; Mammalian; Purification; Receptor; Secreted protein

Year:  2017        PMID： 29075655      PMCID： PMC5654507          DOI： 10.21769/BioProtoc.2430

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  1 in total

1.  IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses.

Authors:  Changchun Chen; Eisuke Itakura; Geoffrey M Nelson; Ming Sheng; Patrick Laurent; Lorenz A Fenk; Rebecca A Butcher; Ramanujan S Hegde; Mario de Bono
Journal:  Nature       Date:  2017-01-18       Impact factor: 49.962
  1 in total
  2 in total

1.  Protocol for quantification of the lysosomal degradation of extracellular proteins into mammalian cells.

Authors:  Ayaka Tomihari; Momoka Chiba; Akira Matsuura; Eisuke Itakura
Journal:  STAR Protoc       Date:  2021-11-23

2.  Heparan sulfate is a clearance receptor for aberrant extracellular proteins.

Authors:  Eisuke Itakura; Momoka Chiba; Takeshi Murata; Akira Matsuura
Journal:  J Cell Biol       Date:  2020-03-02       Impact factor: 10.539
  2 in total

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