Devarati Dutta1, Anindita Sen2, Debkishore Gupta3, Prabir Kuila1, Debasmita Chatterjee4, Sugat Sanyal5, Satadal Das6. 1. Brucella Research Laboratory, Peerless Hospital & B.K. Roy Research Centre, 360 Panchasayar, Kolkata, West Bengal, 700094, India. 2. Department of Microbiology, MGM Medical College, Kishanganj, Bihar, India. 3. Department of Microbiology & Infection Control, CMRI and BM Birla Heart Research Centre, Kolkata, India. 4. Department of Food Technology & Biochemical Engineering, Jadavpur University, Kolkata, India. 5. Department of Laboratory, Peerless Hospital & B.K. Roy Research Centre, Kolkata, India. 6. Brucella Research Laboratory, Peerless Hospital & B.K. Roy Research Centre, 360 Panchasayar, Kolkata, West Bengal, 700094, India. drsatdas@hotmail.com.
Abstract
OBJECTIVE: To investigate the presence of childhood brucellosis presenting as PUO (pyrexia of unknown origin) cases in Eastern zone of India. METHODS: Blood samples were collected from PUO patients aged ≤18 y. The main diagnostic tools were STAT, RBPT, ELISA- IgM, IgG and PCR. Although mainly PUO cases were selected for the study, other associated clinical manifestations were also noted. RESULTS: The findings revealed significantly higher percentage of infection in female children (14.3%) than in male children (10.9%). The positive results by different diagnostic tools, STAT, RBPT, ELISA- IgM, ELISA-IgG and brucella genus specific PCR were 10.6%, 7.2%, 7.2%, 0.85% and 1.3% respectively. Main associated clinical symptoms were joint pain, low backache, fatigue and night sweat. CONCLUSIONS: This hospital based study reflects a significant number of childhood brucellosis cases in Eastern zone of India, and thus emphasizes the need for further monitoring of such subjects.
OBJECTIVE: To investigate the presence of childhood brucellosis presenting as PUO (pyrexia of unknown origin) cases in Eastern zone of India. METHODS: Blood samples were collected from PUO patients aged ≤18 y. The main diagnostic tools were STAT, RBPT, ELISA- IgM, IgG and PCR. Although mainly PUO cases were selected for the study, other associated clinical manifestations were also noted. RESULTS: The findings revealed significantly higher percentage of infection in female children (14.3%) than in male children (10.9%). The positive results by different diagnostic tools, STAT, RBPT, ELISA- IgM, ELISA-IgG and brucella genus specific PCR were 10.6%, 7.2%, 7.2%, 0.85% and 1.3% respectively. Main associated clinical symptoms were joint pain, low backache, fatigue and night sweat. CONCLUSIONS: This hospital based study reflects a significant number of childhood brucellosis cases in Eastern zone of India, and thus emphasizes the need for further monitoring of such subjects.
Entities:
Keywords:
Childhood brucellosis; ELISA; PCR; PUO; RBPT; STAT
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