Literature DB >> 29071286

Caenorhabditis elegans Microinjection.

Matthias Rieckher1, Nektarios Tavernarakis2,3.   

Abstract

Microinjection is the most frequently used tool for genetic transformation of the nematode Caenorhabditis elegans, facilitating the transgenic expression of genes, genome editing by the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, or transcription of dsRNA for RNA intereference (RNAi). Exogenous DNA is delivered into the developing oocytes in the germline of adult hermaphrodites, which then generate transgenic animals among their offspring. In this protocol, we describe the microinjection procedure and the subsequent selection of transgenic progeny.

Entities:  

Keywords:  Caenorhabditis elegans; Extrachromosomal arrays; GFP; Genetic transformation; Germline; Microinjection; Transgenic animals

Year:  2017        PMID： 29071286      PMCID： PMC5734614          DOI： 10.21769/BioProtoc.2565

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  13 in total

1.  A multi-well version of in situ hybridization on whole mount embryos of Caenorhabditis elegans.

Authors:  H Tabara; T Motohashi; Y Kohara
Journal:  Nucleic Acids Res       Date:  1996-06-01       Impact factor: 16.971

2.  Creation of low-copy integrated transgenic lines in Caenorhabditis elegans.

Authors:  V Praitis; E Casey; D Collar; J Austin
Journal:  Genetics       Date:  2001-03       Impact factor: 4.562

3.  pha-1, a selectable marker for gene transfer in C. elegans.

Authors:  M Granato; H Schnabel; R Schnabel
Journal:  Nucleic Acids Res       Date:  1994-05-11       Impact factor: 16.971

Review 4.  DNA transformation.

Authors:  C Mello; A Fire
Journal:  Methods Cell Biol       Date:  1995       Impact factor: 1.441

5.  Green fluorescent protein as a marker for gene expression.

Authors:  M Chalfie; Y Tu; G Euskirchen; W W Ward; D C Prasher
Journal:  Science       Date:  1994-02-11       Impact factor: 47.728

6.  Extrachromosomal DNA transformation of Caenorhabditis elegans.

Authors:  D T Stinchcomb; J E Shaw; S H Carr; D Hirsh
Journal:  Mol Cell Biol       Date:  1985-12       Impact factor: 4.272

7.  Transgenesis in Caenorhabditis elegans.

Authors:  Matthias Rieckher; Nikos Kourtis; Angela Pasparaki; Nektarios Tavernarakis
Journal:  Methods Mol Biol       Date:  2009

8.  Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.

Authors:  C C Mello; J M Kramer; D Stinchcomb; V Ambros
Journal:  EMBO J       Date:  1991-12       Impact factor: 11.598

9.  Single-copy insertion of transgenes in Caenorhabditis elegans.

Authors:  Christian Frøkjaer-Jensen; M Wayne Davis; Christopher E Hopkins; Blake J Newman; Jason M Thummel; Søren-Peter Olesen; Morten Grunnet; Erik M Jorgensen
Journal:  Nat Genet       Date:  2008-10-26       Impact factor: 38.330

Review 10.  CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering.

Authors:  Daniel J Dickinson; Bob Goldstein
Journal:  Genetics       Date:  2016-03       Impact factor: 4.562
View more
  3 in total

1.  Restoration of Proteostasis in the Endoplasmic Reticulum Reverses an Inflammation-Like Response to Cytoplasmic DNA in Caenorhabditis elegans.

Authors:  Ashley B Williams; Felix Heider; Jan-Erik Messling; Matthias Rieckher; Wilhelm Bloch; Björn Schumacher
Journal:  Genetics       Date:  2019-06-27       Impact factor: 4.562

2.  Endogenous formaldehyde scavenges cellular glutathione resulting in redox disruption and cytotoxicity.

Authors:  Carla Umansky; Agustín E Morellato; Matthias Rieckher; Marco A Scheidegger; Manuela R Martinefski; Gabriela A Fernández; Oleg Pak; Ksenia Kolesnikova; Hernán Reingruber; Mariela Bollini; Gerry P Crossan; Natascha Sommer; María Eugenia Monge; Björn Schumacher; Lucas B Pontel
Journal:  Nat Commun       Date:  2022-02-08       Impact factor: 17.694

3.  MINERVA: A CubeSat for demonstrating DNA damage mitigation against space radiation in C. elegans by using genetic modification.

Authors:  Sumeth Klomchitcharoen; Tanchanok Tangwattanasirikun; Sean Gallup; Noparin Smerwong; Peetimon Arunwiriyakit; Pisitchai Tachavises; Jin Tangkijngamwong; Pichamon Phatthanaanukun; Benjamard Jirapanyalerd; Siripak Chattanupakorn; Visarut Rungpongvanich; Norawit Nangsue; Krai Meemon; Patompon Wongtrakoonkate; Suradej Hongeng; Yodchanan Wongsawat
Journal:  Heliyon       Date:  2022-08-17
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.