Liliana Silva1,2,3, Joana Mourão1, Filipa Grosso1, Luísa Peixe1. 1. UCIBIO/REQUIMTE Departamento de Ciências Biológicas Laboratório de Microbiologia Faculdade de Farmácia Universidade do Porto, Porto, Portugal. 2. ESALD, Instituto Politécnico de Castelo Branco, Castelo Branco, Portugal. 3. FEUP Faculdade de Engenharia da Universidade do Porto, Porto, Portugal.
Abstract
OBJECTIVES: Two carbapenemase-carrying plasmids, pLS488 (blaOXA-23) and pLS535 (blaOXA-58) from Acinetobacter pittii clinical isolates, were characterized in this study, including their ability to be transferred to Acinetobacter baumannii. METHODS: The clinical isolates were obtained from drainage fluid of a patient with biliary tract cancer and from an exudate of a patient with a hip infection (Portuguese University Hospital, 2012). Isolate characterization included antimicrobial susceptibility tests, carbapenemase production by Blue-Carba, carbapenem-hydrolysing class D β-lactamase (CHDL) gene search by PCR sequencing, ApaI-PFGE, CHDL genetic location and plasmid size by hybridization and WGS. Plasmid transfer was performed by conjugation or electroporation. RESULTS: pLS488 constitutes the first conjugative plasmid reported to carry a carbapenem resistance gene in A. pittii and is part of a potential new incompatibility group that might also account for the dissemination of OXA-23 in A. baumannii. pLS535 belongs to the Acinetobacter GR7 incompatibility group and presents a new scaffold for OXA-58. This plasmid lacked the machinery for conjugation, but was transferable by electroporation to A. baumannii. Both isolates, which displayed the same PFGE pattern, represent the first report of CHDL-carrying A. pittii in Portuguese hospitals. CONCLUSIONS: Altogether, these results emphasize the importance of A. pittii, or particular A. pittii clones, as a source of resistance genes, facilitating their dissemination among different bacterial species.
OBJECTIVES: Two carbapenemase-carrying plasmids, pLS488 (blaOXA-23) and pLS535 (blaOXA-58) from Acinetobacter pittii clinical isolates, were characterized in this study, including their ability to be transferred to Acinetobacter baumannii. METHODS: The clinical isolates were obtained from drainage fluid of a patient with biliary tract cancer and from an exudate of a patient with a hip infection (Portuguese University Hospital, 2012). Isolate characterization included antimicrobial susceptibility tests, carbapenemase production by Blue-Carba, carbapenem-hydrolysing class D β-lactamase (CHDL) gene search by PCR sequencing, ApaI-PFGE, CHDL genetic location and plasmid size by hybridization and WGS. Plasmid transfer was performed by conjugation or electroporation. RESULTS: pLS488 constitutes the first conjugative plasmid reported to carry a carbapenem resistance gene in A. pittii and is part of a potential new incompatibility group that might also account for the dissemination of OXA-23 in A. baumannii. pLS535 belongs to the Acinetobacter GR7 incompatibility group and presents a new scaffold for OXA-58. This plasmid lacked the machinery for conjugation, but was transferable by electroporation to A. baumannii. Both isolates, which displayed the same PFGE pattern, represent the first report of CHDL-carrying A. pittii in Portuguese hospitals. CONCLUSIONS: Altogether, these results emphasize the importance of A. pittii, or particular A. pittii clones, as a source of resistance genes, facilitating their dissemination among different bacterial species.
Authors: Adriana P Matos; Rodrigo Cayô; Luiz G P Almeida; Ana Paula Streling; Carolina S Nodari; Willames M B S Martins; Ana Clara Narciso; Rosa M Silva; Ana T R Vasconcelos; Ana C Gales Journal: mSphere Date: 2019-10-16 Impact factor: 4.389
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Authors: Jetsi Mancilla-Rojano; Sara A Ochoa; Juan Pablo Reyes-Grajeda; Víctor Flores; Oscar Medina-Contreras; Karina Espinosa-Mazariego; Israel Parra-Ortega; Daniela De La Rosa-Zamboni; María Del Carmen Castellanos-Cruz; José Arellano-Galindo; Miguel A Cevallos; Rigoberto Hernández-Castro; Juan Xicohtencatl-Cortes; Ariadnna Cruz-Córdova Journal: Front Microbiol Date: 2020-10-15 Impact factor: 5.640