J Kaplon1, N Grangier2, S Pillet3, A Minoui-Tran4, A Vabret5, N Wilhelm6, N Prieur7, M Lazrek8, S Alain9, Y Mekki10, V Foulongne11, J Guinard12, V Avettand-Fenoel13, A Schnuriger13, A Beby-Defaux14, G Lagathu15, P Pothier2, A de Rougemont16. 1. National Reference Centre for Gastroenteritis Viruses, Laboratory of Biology and Pathology, University Hospital of Dijon, France; UMR PAM A 02.102 Procédés Alimentaires et Microbiologiques, Université de Bourgogne Franche-Comté/AgroSup Dijon, France; French Rotavirus Network, France. Electronic address: jerome.kaplon@chu-dijon.fr. 2. National Reference Centre for Gastroenteritis Viruses, Laboratory of Biology and Pathology, University Hospital of Dijon, France; French Rotavirus Network, France. 3. French Rotavirus Network, France; Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, France. 4. French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Brest, France. 5. French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Caen, France. 6. French Rotavirus Network, France; Laboratory of Clinical Biology, Hospital of Cahors, France. 7. French Rotavirus Network, France; Laboratory of Clinical Biology, Hospital of Charleville-Mézières, France. 8. French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Lille, France. 9. French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Limoges, France. 10. French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Lyon, France. 11. French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Montpellier, France. 12. French Rotavirus Network, France; Laboratory of Clinical Biology, Hospital of Orléans, France. 13. French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Paris, France. 14. French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Poitiers, France. 15. French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Rennes, France. 16. National Reference Centre for Gastroenteritis Viruses, Laboratory of Biology and Pathology, University Hospital of Dijon, France; UMR PAM A 02.102 Procédés Alimentaires et Microbiologiques, Université de Bourgogne Franche-Comté/AgroSup Dijon, France; French Rotavirus Network, France. Electronic address: alexis.de-rougemont@u-bourgogne.fr.
Abstract
OBJECTIVES: Group A rotavirus is a major cause of acute gastroenteritis in young children worldwide. A prospective surveillance network has been set up in France to investigate rotavirus infections and to detect the emergence of potentially epidemic strains. METHODS: From 2014 to 2017, rotavirus-positive stool samples were collected from 2394 children under 5 years old attending the paediatric emergency units of 13 large hospitals. Rotaviruses were genotyped by RT-PCR with regard to their outer capsid proteins VP4 and VP7. RESULTS: Genotyping of 2421 rotaviruses showed that after a marked increase in G9P[8] (32.1%) during the 2014-2015 season, G9P[8] became the predominant genotype during the 2015-2016 and 2016-2017 seasons with detection rates of 64.1% and 77.3%, respectively, whereas G1P[8] were detected at low rates of 16.8% and 6.6%, respectively. Phylogenetic analysis of the partial rotavirus VP7 and VP4 coding genes revealed that all of these G9P [8] strains belonged to the lineage III and the P [8]-3 lineage, respectively, and shared the same genetic background (G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) as did most of previously detected G9P[8] strains and particularly the emerging G9P[8] strains from the 2004-2005 season in France. CONCLUSIONS: G9P[8] rotaviruses have become the predominant circulating genotype for the first time since their emergence a decade ago. In the absence of rotavirus immunization programmes in France, our data give an insight into the natural fluctuation of rotavirus genotypes in a non-vaccinated population and provide a base line for a better interpretation of data in European countries with routine rotavirus vaccination.
OBJECTIVES:Group A rotavirus is a major cause of acute gastroenteritis in young children worldwide. A prospective surveillance network has been set up in France to investigate rotavirus infections and to detect the emergence of potentially epidemic strains. METHODS: From 2014 to 2017, rotavirus-positive stool samples were collected from 2394 children under 5 years old attending the paediatric emergency units of 13 large hospitals. Rotaviruses were genotyped by RT-PCR with regard to their outer capsid proteins VP4 and VP7. RESULTS: Genotyping of 2421 rotaviruses showed that after a marked increase in G9P[8] (32.1%) during the 2014-2015 season, G9P[8] became the predominant genotype during the 2015-2016 and 2016-2017 seasons with detection rates of 64.1% and 77.3%, respectively, whereas G1P[8] were detected at low rates of 16.8% and 6.6%, respectively. Phylogenetic analysis of the partial rotavirus VP7 and VP4 coding genes revealed that all of these G9P [8] strains belonged to the lineage III and the P [8]-3 lineage, respectively, and shared the same genetic background (G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) as did most of previously detected G9P[8] strains and particularly the emerging G9P[8] strains from the 2004-2005 season in France. CONCLUSIONS: G9P[8] rotaviruses have become the predominant circulating genotype for the first time since their emergence a decade ago. In the absence of rotavirus immunization programmes in France, our data give an insight into the natural fluctuation of rotavirus genotypes in a non-vaccinated population and provide a base line for a better interpretation of data in European countries with routine rotavirus vaccination.
Authors: Chiara de Waure; Laura Sarnari; Manuela Chiavarini; Giovanni Ianiro; Marina Monini; Anna Alunno; Barbara Camilloni Journal: Int J Environ Res Public Health Date: 2020-02-05 Impact factor: 3.390